Nathalang Oytip, Asisathaporn Kamonchanok, Intharanut Kamphon, Chaibangyang Wanlapa, Leetrakool Nipapan, Mitundee Supattra, Bejrachandra Sasitorn
Graduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand.
Blood Bank Section, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
J Blood Med. 2023 Feb 17;14:133-140. doi: 10.2147/JBM.S398720. eCollection 2023.
Co and Co antigens of the Colton (CO) blood group system are implicated in acute and delayed hemolytic transfusion reactions (HTRs). Owing to the inadequate supply of specific antiserum, data on CO phenotypes remain limited. This study aimed to develop genotyping methods to predict Co and Co antigens and to estimate transfusion-induced alloimmunization risks in three Thai blood donor populations.
The study included 2451 blood samples from unrelated healthy Thai blood donors obtained from central, northern, and southern Thailand. DNA sequencing was used to determine the and alleles. In-house PCR with sequence-specific primers (PCR-SSP) and high-resolution melting curve (HRM) assays were performed and genotyping results were compared using DNA sequencing. and allele frequencies among Thais were determined using PCR-SSP and their frequencies were compared with other populations. The risks of Co and Co transfusion-induced alloimmunization among Thai donor populations were calculated.
The validated genotyping results by PCR-SSP and HRM assays agreed with DNA sequencing. The / was the most common (100.0, 100.0, and 99.3%), followed by / (0.0, 0.0, and 0.7%) among central, northern and southern Thais. Homozygous / was not found. The and allele frequencies among central Thais significantly differed compared among southern Thais ( < 0.01) but not among northern Thais. Those allele frequencies among Thais were similar to those of Taiwanese, Chinese and Malay-Malaysian populations but not to South Asian, Southeast Asian, Korean, Japanese, Filipino, French Basque, and Maltese populations ( < 0.01). A higher risk of anti-Co production rather than anti-Co production was particularly noted in the southern Thai population.
This study constitutes the first to determine and genotypes using PCR-SSP and HRM assays among Thais and this finding would be beneficial in predicting alloimmunization risk and providing safe transfusions among Thais.
科尔顿（CO）血型系统的Co和Co抗原与急性和迟发性溶血性输血反应（HTR）有关。由于特异性抗血清供应不足，关于CO血型表型的数据仍然有限。本研究旨在开发基因分型方法，以预测Co和Co抗原，并估计泰国三个献血者群体中输血诱导的同种免疫风险。
本研究纳入了2451份来自泰国中部、北部和南部无关健康献血者的血样。采用DNA测序法确定 和 等位基因。进行了基于序列特异性引物的内部PCR（PCR-SSP）和高分辨率熔解曲线（HRM）分析，并将基因分型结果与DNA测序结果进行比较。使用PCR-SSP确定泰国人中 和 等位基因频率，并将其频率与其他人群进行比较。计算泰国献血者群体中Co和Co输血诱导同种免疫的风险。
PCR-SSP和HRM分析验证的基因分型结果与DNA测序结果一致。在泰国中部、北部和南部人群中， / 最为常见（分别为100.0%、100.0%和99.3%），其次是 / （分别为0.0%、0.0%和0.7%）。未发现纯合子 / 。泰国中部人群中的 和 等位基因频率与泰国南部人群相比有显著差异（ < 0.01），但与泰国北部人群无差异。泰国人群中的这些等位基因频率与台湾、中国和马来-马来西亚人群相似，但与南亚、东南亚、韩国、日本、菲律宾、法国巴斯克和马耳他人群不同（ < 0.01）。特别值得注意的是，泰国南部人群中产生抗Co的风险高于产生抗Co的风险。
本研究首次在泰国人群中使用PCR-SSP和HRM分析确定了 和 基因型，这一发现将有助于预测同种免疫风险，并在泰国人群中提供安全输血。
