Vladimirsky M A, Shipina L K, Makeeva E S, Alyapkina Y S, Mikheev A Y, Morozov V N
Phthisiopulmonology Research Institute of the Sechenov First State Medical University, Moscow, Russian Federation.
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 3, Institutskaya Str., Moscow Region, Pushchino, Russian Federation.
J Hosp Infect. 2016 May;93(1):100-4. doi: 10.1016/j.jhin.2016.02.009. Epub 2016 Mar 3.
A simple inexpensive technique for collection of airborne biomarkers of nosocomial infections is described. Biomarkers were collected on water-soluble electrospun nanofilters attached to a household vacuum cleaner from 6-10m(3) of air in 10-15min within several wards of a tuberculosis clinic. Filters were then dissolved in water and tested for the presence of the IS6110 and regX3 genes of Mycobacterium tuberculosis (MTB) using real-time polymerase chain reaction. It was demonstrated that trace amounts of airborne MTB DNA (>3gene copies/m(3)) were always present in the air and on the surfaces in the wards occupied with tuberculosis patients having positive smear tests.
描述了一种用于收集医院感染空气传播生物标志物的简单廉价技术。在结核病诊所的几个病房内，通过连接到家用吸尘器的水溶性电纺纳米过滤器，在10 - 15分钟内从6 - 10立方米空气中收集生物标志物。然后将过滤器溶解在水中，并使用实时聚合酶链反应检测结核分枝杆菌（MTB）的IS6110和regX3基因的存在。结果表明，在涂片检查呈阳性的结核病患者所在病房的空气中和表面总是存在痕量的空气传播MTB DNA（>3个基因拷贝/立方米）。
