Chen Lei, Ai Peng, Zhang Jinfeng, Deng Qiming, Wang Shiquan, Li Shuangcheng, Zhu Jun, Li Ping, Zheng Aiping
Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, ChinaKey laboratory of Sichuan Crop Major Disease, Sichuan Agricultural University, Chengdu, 611130, ChinaKey Laboratory of Southwest Crop Gene Resource and Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, 625014, China
Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, ChinaKey laboratory of Sichuan Crop Major Disease, Sichuan Agricultural University, Chengdu, 611130, ChinaKey Laboratory of Southwest Crop Gene Resource and Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, 625014, China.
Database (Oxford). 2016 Mar 28;2016. doi: 10.1093/database/baw031. Print 2016.
Rice [Oryza sativa (L.)] feeds more than half of the world's population. Rhizoctonia solaniis a major fungal pathogen of rice causing extreme crop losses in all rice-growing regions of the world. R. solani AG1 IA is a major cause of sheath blight in rice. In this study, we constructed a comprehensive and user-friendly web-based database, RSIADB, to analyse its draft genome and transcriptome. The database was built using the genome sequence (10,489 genes) and annotation information for R. solani AG1 IA. A total of six RNAseq samples of R. solani AG1 IA were also analysed, corresponding to 10, 18, 24, 32, 48 and 72 h after infection of rice leaves. The RSIADB database enables users to search, browse, and download gene sequences for R. solani AG1 IA, and mine the data using BLAST, Sequence Extractor, Browse and Construction Diagram tools that were integrated into the database. RSIADB is an important genomic resource for scientists working with R. solani AG1 IA and will assist researchers in analysing the annotated genome and transcriptome of this pathogen. This resource will facilitate studies on gene function, pathogenesis factors and secreted proteins, as well as provide an avenue for comparative analyses of genes expressed during different stages of infection. Database URL:http://genedenovoweb.ticp.net:81/rsia/index.php.
水稻[Oryza sativa (L.)]养活了世界上一半以上的人口。立枯丝核菌是水稻的一种主要真菌病原体，在世界所有水稻种植地区都会导致严重的作物损失。立枯丝核菌AG1 IA是水稻纹枯病的主要病因。在本研究中，我们构建了一个全面且用户友好的基于网络的数据库RSIADB，用于分析其基因组草图和转录组。该数据库是利用立枯丝核菌AG1 IA的基因组序列（10,489个基因）和注释信息构建的。我们还分析了立枯丝核菌AG1 IA的总共六个RNAseq样本，分别对应于感染水稻叶片后10、18、24、32、48和72小时。RSIADB数据库使用户能够搜索、浏览和下载立枯丝核菌AG1 IA的基因序列，并使用集成到数据库中的BLAST、序列提取器、浏览和构建图工具挖掘数据。RSIADB是研究立枯丝核菌AG1 IA的科学家的重要基因组资源，将有助于研究人员分析该病原体的注释基因组和转录组。该资源将促进对基因功能、致病因子和分泌蛋白的研究，并为分析感染不同阶段表达的基因提供比较分析途径。数据库网址：http://genedenovoweb.ticp.net:81/rsia/index.php。
