Prashantha S T, Bashyal B M, Krishnan S Gopala, Dubey Himanshu, Solanke Amolkumar U, Prakash G, Aggarwal Rashmi
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.
3 Biotech. 2021 Aug;11(8):394. doi: 10.1007/s13205-021-02934-1. Epub 2021 Aug 2.
Sheath blight disease caused by Kuhn (teleomorph; ) is a major constraint in rice production. Among the different anastomosis groups (AGs) of , AG1-IA causes sheath blight of rice, which induce necrotic lesions on leaf sheaths of the infected plants. Several reports contradict the host specificity of anastomosis groups in . There is lack of information on the pathogenicity genes of these anastomosis groups during sheath blight infection in rice. In the present study, isolates collected from diverse rice growing regions of India were screened for anastomosis groups and two groups namely, AG1-IA, AG2-2 were identified. Accordingly, comparative studies were made with AG1-IA (GenBank ID: 16,395) and AG2-2 (GenBank ID: 2,318,768) group sequences, which enabled the identification of specific gene clusters (119 in AG1-IA and 604 in AG2-2) belonging to these groups. Pathogen Host Interaction (PHI) blast with these specific gene clusters could further identify genes involved in host pathogen interaction (38 in AG1_IA and 150 in AG2-2), which were shortlisted for qRT-PCR validation based on qcov cutoff values representing different phenotypic categories of PHI blast. Expression analysis-based validation in sheath blight susceptible (Pusa Basmati 1) and resistant (Pusa 1908-13-12-5) rice genotypes showed that most of the genes expressed significantly higher in the susceptible variety Pusa Basmati 1. The genes like inorganic phosphate transporter (), Bromodomain containing protein (), Aldehyde dehydrogenase (), AMP binding domain () and Heme peroxidase () were upregulated in the susceptible genotype, PB 1 at 72hpi compared to Pusa 1908-13-12-5. Among these, inorganic phosphate transporter (), Bromodomain containing protein () and Heme peroxidase () were specific to AG1-IA. Through the present study, we could demonstrate the AG1-IA-specific interactions of causing sheath blight disease of rice, which is a step forward in understanding the specificity of with reference to sheath blight disease of rice.
The online version contains supplementary material available at 10.1007/s13205-021-02934-1.
由库恩氏菌（有性型； ）引起的纹枯病是水稻生产中的主要限制因素。在库恩氏菌的不同融合群（AGs）中，AG1-IA会引发水稻纹枯病，在受感染植株的叶鞘上诱发坏死病斑。有几份报告与库恩氏菌融合群的寄主特异性相矛盾。关于这些库恩氏菌融合群在水稻纹枯病感染过程中的致病基因，目前缺乏相关信息。在本研究中，对从印度不同水稻种植区收集的库恩氏菌分离株进行了融合群筛选，鉴定出了两个融合群，即AG1-IA和AG2-2。据此，对AG1-IA（GenBank登录号：16,395）和AG2-2（GenBank登录号：2,318,768）群序列进行了比较研究，从而鉴定出属于这些群的特定基因簇（AG1-IA中有119个，AG2-2中有604个）。用这些特定基因簇进行病原菌-寄主互作（PHI）比对，可进一步鉴定出参与寄主-病原菌互作的基因（AG1_IA中有38个，AG2-2中有150个），根据代表PHI比对不同表型类别的qcov截止值，将这些基因列入qRT-PCR验证的候选名单。在纹枯病感病（Pusa Basmati 1）和抗病（Pusa 1908-13-12-5）水稻基因型中基于表达分析的验证表明，大多数基因在感病品种Pusa Basmati 1中的表达显著更高。与Pusa 1908-13-12-5相比，无机磷酸盐转运蛋白（ ）、含溴结构域蛋白（ ）、醛脱氢酶（ ）、AMP结合结构域（ ）和血红素过氧化物酶（ ）等基因在感病基因型PB 1中于接种后72小时上调。其中，无机磷酸盐转运蛋白（ ）、含溴结构域蛋白（ ）和血红素过氧化物酶（ ）是AG1-IA特有的。通过本研究，我们能够证明引起水稻纹枯病的库恩氏菌与AG1-IA的特异性相互作用，这在理解库恩氏菌与水稻纹枯病的特异性方面向前迈进了一步。
在线版本包含可在10.1007/s13205-021-02934-1获取的补充材料。
