Cardoso Tulio Pereira, de Rezende Duek Eliana Aparecida, Amatuzzi Marco Martins, Caetano Edie Benedito
PhD from the School of Medicine of the University of Sao Paulo; Collaborating Research in the Biomaterials Laboratory, Medical and Biological Sciences Center of Sorocaba, Catholic Pontificate University of Sao Paulo.
Titular Professor of the Department of Biology; Head of the Biomaterials Laboratory, Medical and Biological Sciences Center of Sorocaba, Catholic Pontificate University of Sao Paulo.
Rev Bras Ortop. 2015 Nov 17;45(3):247-59. doi: 10.1016/S2255-4971(15)30365-7. eCollection 2010 May-Jun.
To induce growth of a neomeniscus into the pores of a prosthesis in order to protect the knee joint cartilage.
70 knees of 35 New Zealand rabbits were operated. The rabbits were five to seven months old, weighed 2 to 3.8 kilograms, and 22 were male and 13 were female. Each animal underwent medial meniscectomy in both knees during a single operation. A bioabsorbable polymeric meniscal prosthesis composed of 70% polydioxanone and 30% L-lactic acid polymer was implanted in one side. The animals were sacrificed after different postoperative time intervals. The femoral condyles and neomeniscus were subjected to histological analysis. Histograms were used to measure the degradation and absorption of the prosthesis, the growth of meniscal tissue in the prosthesis and the degree of degradation of the femoral condyle joint cartilage.
The data obtained showed that tissue growth histologically resembling a normal meniscus occurred, with gradual absorption of the prosthesis, and the percentages of chondrocytes on the control side and prosthesis side.
Tissue growth into the prosthesis pores that histologically resembled the normal rabbit meniscus was observed. The joint cartilage of the femoral condyles on the prosthesis side presented greater numbers of chondrocytes in all its layers.
诱导新生半月板长入假体孔隙以保护膝关节软骨。
对35只新西兰兔的70个膝关节进行手术。兔子年龄为5至7个月，体重2至3.8千克，其中雄性22只，雌性13只。每只动物在一次手术中双侧膝关节均行内侧半月板切除术。一侧植入由70%聚二氧六环酮和30%L-乳酸聚合物组成的可生物吸收的聚合物半月板假体。在术后不同时间间隔处死动物。对股骨髁和新生半月板进行组织学分析。用直方图测量假体的降解和吸收情况、半月板组织在假体中的生长情况以及股骨髁关节软骨的降解程度。
所获数据显示，组织学上出现了类似正常半月板的生长，假体逐渐吸收，以及对照侧和假体侧软骨细胞的百分比。
观察到组织长入假体孔隙，组织学上类似于正常兔半月板。假体侧股骨髁的关节软骨各层均有较多的软骨细胞。
