Cook J L, Tomlinson J L, Kreeger J M, Cook C R
University of Missouri-Columbia, Veterinary Medical Teaching Hospital, MO 65211, USA.
Am J Sports Med. 1999 Sep-Oct;27(5):658-65. doi: 10.1177/03635465990270051901.
A unique biomaterial, porcine small intestinal submucosa, was used to construct grafts for implantation into surgically created medial meniscal defects in dogs. Five dogs received grafts and two were left untreated as controls. All dogs were evaluated at 4, 8, and 12 weeks by means of lameness scoring, force plate analysis, and ultrasonography. Twelve weeks after implantation the dogs were sacrificed and the replacement tissue was evaluated for gross and histologic appearance, amount, glycosaminoglycan content, and type II collagen immunoreactivity. Four weeks after instrumentation, both groups had lameness scores that were significantly higher than preoperative scores, but at the 8- and 12-week evaluations, scores for the grafted dogs were not different from preoperative values. The ultrasonographic appearance of replacement tissue in grafted defects resembled normal meniscus. In the untreated defects, only unorganized tissue was present. In control dogs, replacement tissue resembled fibrous tissue and cartilage erosions were visible on the medial femoral condyles. In four of the five grafted dogs, replacement tissue was grossly indistinguishable from normal meniscus. The amount of tissue in the defect was significantly greater for the grafted dogs. Histologically, replacement tissue in control dogs was composed of vascularized connective tissue with no evidence of chondroid differentiation. Replacement tissue in grafted dogs closely resembled normal meniscal tissue with respect to chondroid differentiation, collagen content, and zonal architecture. Porcine small intestinal submucosa appeared to have beneficial effects on meniscal regeneration.
一种独特的生物材料——猪小肠黏膜下层，被用于构建移植体，植入通过手术制造的犬内侧半月板缺损处。五只犬接受了移植体，两只未接受治疗作为对照。所有犬在4周、8周和12周时通过跛行评分、测力板分析和超声检查进行评估。植入12周后，处死犬并对替代组织进行大体和组织学外观、数量、糖胺聚糖含量及II型胶原免疫反应性评估。器械植入4周后，两组的跛行评分均显著高于术前评分，但在8周和12周评估时，接受移植体的犬的评分与术前值无差异。移植缺损处替代组织的超声表现类似于正常半月板。在未治疗的缺损处，仅存在无组织的组织。在对照犬中，替代组织类似于纤维组织，内侧股骨髁可见软骨侵蚀。在五只接受移植体的犬中的四只，替代组织在大体上与正常半月板无法区分。接受移植体的犬缺损处的组织量显著更大。组织学上，对照犬的替代组织由血管化结缔组织组成，无软骨样分化证据。接受移植体的犬的替代组织在软骨样分化、胶原含量和区域结构方面与正常半月板组织非常相似。猪小肠黏膜下层似乎对半月板再生有有益作用。
