Song Eun-Hye, Kim Hyun-Ju, Jeong Jaesik, Chung Hyun-Jung, Kim Han-Yong, Bang Eunjung, Hong Young-Shick
Division of Food and Nutrition, Chonnam National University , Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
Western Seoul Center, Korea Basic Science Institute , Seoul 136-701, Republic of Korea.
J Agric Food Chem. 2016 Apr 20;64(15):3009-16. doi: 10.1021/acs.jafc.5b05667. Epub 2016 Apr 6.
Rice grain metabolites are important for better understanding of the plant physiology of various rice cultivars and thus for developing rice cultivars aimed at providing diverse processed products. However, the variation of global metabolites in rice grains has rarely been explored. Here, we report the identification of intra- or intercellular metabolites in rice (Oryza sativa L.) grain powder using a (1)H high-resolution magic angle spinning (HR-MAS) NMR-based metabolomic approach. Compared with nonwaxy rice cultivars, marked accumulation of lipid metabolites such as fatty acids, phospholipids, and glycerophosphocholine in the grains of waxy rice cultivars demonstrated the distinct metabolic regulation and adaptation of each cultivar for effective growth during future germination, which may be reflected by high levels of glutamate, aspartate, asparagine, alanine, and sucrose. Therefore, this study provides important insights into the metabolic variations of diverse rice cultivars and their associations with environmental conditions and genetic backgrounds, with the aim of facilitating efficient development and the improvement of rice grain quality through inbreeding with genetic or chemical modification and mutation.
水稻籽粒代谢产物对于更好地理解各种水稻品种的植物生理学至关重要，因此对于培育旨在提供多样化加工产品的水稻品种也很重要。然而，水稻籽粒中全球代谢产物的变化很少被探究。在此，我们报告了使用基于¹H高分辨率魔角旋转（HR-MAS）核磁共振的代谢组学方法对水稻（Oryza sativa L.）籽粒粉末中的细胞内或细胞间代谢产物进行鉴定。与非糯稻品种相比，糯稻品种籽粒中脂肪酸、磷脂和甘油磷酸胆碱等脂质代谢产物的显著积累表明了每个品种在未来萌发期间有效生长的独特代谢调控和适应性，这可能通过高水平的谷氨酸、天冬氨酸、天冬酰胺、丙氨酸和蔗糖来体现。因此，本研究为不同水稻品种的代谢变化及其与环境条件和遗传背景的关联提供了重要见解，旨在通过基因或化学修饰和诱变的近亲繁殖促进水稻籽粒品质的高效发育和改善。
