Coulthard Alistair B, Taylor-Kamall Rhodri W, Hallson Graham, Axentiev Anna, Sinclair Don A, Honda Barry M, Hilliker Arthur J
a Department of Biology, York University, Toronto, ON M3J 1P3, Canada.
b Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
Genome. 2016 Apr;59(4):289-94. doi: 10.1139/gen-2015-0171.
In Drosophila melanogaster, the borders between pericentric heterochromatin and euchromatin on the major chromosome arms have been defined in various ways, including chromatin-specific histone modifications, the binding patterns of heterochromatin-enriched chromosomal proteins, and various cytogenetic techniques. Elucidation of the genetic properties that independently define the different chromatin states associated with heterochromatin and euchromatin should help refine the boundary. Since meiotic recombination is present in euchromatin, but absent in heterochromatin, it constitutes a key genetic property that can be observed transitioning between chromatin states. Using P element insertion lines marked with a su(Hw) insulated mini-white gene, meiotic recombination was found to transition in a region consistent with the H3K9me2 transition observed in ovaries.
在黑腹果蝇中，主要染色体臂上的着丝粒周围异染色质和常染色质之间的边界已通过多种方式定义，包括染色质特异性组蛋白修饰、富含异染色质的染色体蛋白的结合模式以及各种细胞遗传学技术。阐明独立定义与异染色质和常染色质相关的不同染色质状态的遗传特性，应有助于完善边界。由于减数分裂重组存在于常染色质中，而不存在于异染色质中，它构成了一种关键的遗传特性，可以观察到其在染色质状态之间转变。使用标记有su(Hw)绝缘小白色基因的P因子插入系，发现减数分裂重组在与卵巢中观察到的H3K9me2转变一致的区域发生转变。
