基质金属蛋白酶-9在口腔鳞状细胞癌中的预后价值及其与血管生成的关系。
Prognostic value of matrix metalloproteinase-9 expression in oral squamous cell carcinoma and its association with angiogenesis.
作者信息
Andisheh-Tadbir Azadeh, Mardani Maryam, Pourshahidi Sara, Nezarati Kamran, Bahadori Parisa
机构信息
Associate Professor, Prevention of Oral and Dental Disease Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Assistant Professor, Department of Oral Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
J Clin Exp Dent. 2016 Apr 1;8(2):e130-5. doi: 10.4317/jced.52712. eCollection 2016 Apr.
BACKGROUND
Breakdown of extracellular matrix (ECM) is one of the important hallmarks of cancer progression which facilitates the invasion of tumoral cells to the surrounding tissue. Matrix metalloproteinases (MMPs) can degrade various components of the ECM and basement membrane. The aim of this study was to determine the role of matrix metalloproteinases-9 protein in the biologic behavior of oral squamous cell carcinoma (OSCC) and its relation with tumor angiogenesis.
MATERIAL AND METHODS
In this study 42 OSCC and 15 normal epithelium were reviewed by immunohistochemical staining for matrix metalloproteinases-9 and CD105.
RESULTS
Matrix metalloproteinases-9 expression was detected in 32 OSCC specimens (76.1%), with 28 specimens (66.6%) showing moderate or strong expression. We observed that the expression level of matrix metalloproteinases-9 was positively correlated with the status of lymph node metastasis (N0vs. N1) (P =0.00), and clinical stage (I-II vs. III-IV) in OSCC patients. Microvessel density in intratumoral tissue has an association with lymph node metastasis and advanced clinical stage (P=0.003 and p=0.01, respectively). We observed that tumors with matrix metalloproteinases-9 overexpression had a higher microvessel density counts compared with tumors with absent or focal immunostaining(16.2±5.6 vs 10.3±3.5 respectively, P =0.03).
CONCLUSIONS
In conclusion present results demonstrate the marked expression of matrix metalloproteinases-9 and CD105 in OSCC and suggest that the expression of these markers is associated with tumor progression and could offer additional information about the aggressiveness of OSCC. In addition a significant relationship was noted between microvessel density count and expression of matrix metalloproteinases-9 which suggest that MMP9 expression may be closely related to tumor angiogenesis.
KEY WORDS
Matrix metalloproteinases-9, CD105, squamous cell carcinoma, immunohistochemistry.
背景
细胞外基质（ECM）的降解是癌症进展的重要标志之一，它促进肿瘤细胞向周围组织浸润。基质金属蛋白酶（MMPs）能够降解ECM和基底膜的各种成分。本研究旨在确定基质金属蛋白酶-9蛋白在口腔鳞状细胞癌（OSCC）生物学行为中的作用及其与肿瘤血管生成的关系。
材料与方法
本研究通过免疫组织化学染色检测42例OSCC和15例正常上皮组织中的基质金属蛋白酶-9和CD105。
结果
在32例OSCC标本（76.1%）中检测到基质金属蛋白酶-9表达，其中28例标本（66.6%）呈中度或强表达。我们观察到，OSCC患者中基质金属蛋白酶-9的表达水平与淋巴结转移状态（N0 vs. N1）（P =0.00）及临床分期（I-II期vs. III-IV期）呈正相关。肿瘤组织内微血管密度与淋巴结转移及临床晚期相关（分别为P=0.003和P=0.01）。我们观察到，与无或局灶免疫染色的肿瘤相比，基质金属蛋白酶-9过表达的肿瘤微血管密度计数更高（分别为16.2±5.6 vs 10.3±3.5，P =0.03）。
结论
总之，目前的结果表明基质金属蛋白酶-9和CD105在OSCC中显著表达，提示这些标志物的表达与肿瘤进展相关，并可为OSCC的侵袭性提供额外信息。此外，微血管密度计数与基质金属蛋白酶-9的表达之间存在显著关系，这表明MMP9表达可能与肿瘤血管生成密切相关。
关键词
基质金属蛋白酶-9；CD105；鳞状细胞癌；免疫组织化学
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