Silva Eline Manhães Reid, Freitas Vanessa Morais, Bautz Willian Grassi, de Barros Liliana Aparecida Pimenta, da Gama de Souza Letícia Nogueira
Dental Clinic, Federal University of Espírito Santo, Health of Sciences CenterBrazil.
Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São PauloBrazil.
J Oral Maxillofac Res. 2018 Mar 31;9(1):e3. doi: 10.5037/jomr.2018.9103. eCollection 2018 Jan-Mar.
Oral squamous cell carcinoma is associated with alterations in basement membrane. Laminin-332 is present in basal lamina and performs multiple biologic effects by γ2 chain. Matrix metalloproteinase acts disrupting extracellular components and was related to poor prognosis in cancer. Here, molecular profile of laminin-332 γ2 chain and matrix metalloproteinase-9 was assessed in oral lesions.
The expression of laminin-332 γ2 chain and matrix metalloproteinase-9 (MMP-9) was examined by immunohistochemistry in 10 patients with high risk of malignant transformation oral lesions and 26 cases of oral squamous cell carcinoma (OSCC). Associations between microscopic and clinicopathologic features were established.
Immunostaining of laminin-332 γ2 chain in high risk oral lesions was most detected in basement membrane which is continuous, while the majority of OSCC cases showed a discontinuous membrane (P = 0.001). It was observed a positive reaction for γ2 chain in invasive fronts and a higher expression in epithelial compartment of smoking patients with OSCC (P < 0.0001). In epithelium, MMP-9 expression was presented in all layers with no difference between lesions. However, an elevated immunostaining in stromal cells was associated with male patients (P = 0.0054), older than 60 years (P = 0.0101) and with OSCC.
Present study results support the hypothesis of changes in molecules expression in high risk oral lesions and oral squamous cell carcinoma. A relation between clinical and molecule profile was observed. Those molecules may represent a useful tool to predict oral cancer behaviour.
口腔鳞状细胞癌与基底膜改变有关。层粘连蛋白-332存在于基膜中，并通过γ2链发挥多种生物学作用。基质金属蛋白酶可破坏细胞外成分，与癌症预后不良有关。在此，对口腔病变中层粘连蛋白-332γ2链和基质金属蛋白酶-9的分子特征进行评估。
采用免疫组织化学法检测10例有恶变高危口腔病变患者和26例口腔鳞状细胞癌（OSCC）患者中层粘连蛋白-332γ2链和基质金属蛋白酶-9（MMP-9）的表达。建立微观特征与临床病理特征之间的关联。
高危口腔病变中层粘连蛋白-332γ2链的免疫染色在连续的基底膜中最易检测到，而大多数OSCC病例显示膜不连续（P = 0.001）。在侵袭前沿观察到γ2链呈阳性反应，且在吸烟的OSCC患者上皮区表达较高（P < 0.0001）。在上皮中，MMP-9在所有层均有表达，病变之间无差异。然而，基质细胞中免疫染色升高与男性患者（P = 0.0054）、年龄大于60岁（P = 0.0101）以及OSCC有关。
本研究结果支持高危口腔病变和口腔鳞状细胞癌中分子表达发生变化的假说。观察到临床特征与分子特征之间存在关联。这些分子可能是预测口腔癌行为的有用工具。
