Waters H M, Smith C, Howarth J E, Dawson D W, Delamore I W
University Department of Haematology, Manchester Royal Infirmary.
J Clin Pathol. 1989 Mar;42(3):307-12. doi: 10.1136/jcp.42.3.307.
A method for the detection of total, type I, and type II intrinsic factor antibodies was devised. The technique comprises a two-site solid phase enzyme linked immunosorbent assay (ELISA), with human intrinsic factor conjugated with horseradish peroxidase as label and attached to polystyrene tubes as solid phase. One conjugation provides sufficient material to assay more than 10,000 patient samples. The label proved stable during the course of this evaluation and was still in use more than 12 months after preparation. When applied to 45 serum samples from cases of pernicious anaemia, intrinsic factor antibodies were shown in 30 (67%). Simplicity, high capacity, low cost and label stability, combined with relatively high clinical sensitivity make the method suitable for cost effective screening of large numbers of samples. Simple modifications to the basic assay reagents permitted type I and type II intrinsic factor antibodies to be differentiated.
设计了一种检测总内因子抗体、I型内因子抗体和II型内因子抗体的方法。该技术包括双位点固相酶联免疫吸附测定（ELISA），以与辣根过氧化物酶结合的人内因子作为标记物，并附着于聚苯乙烯管作为固相。一次结合所提供的材料足以检测10000多个患者样本。在本次评估过程中，该标记物被证明是稳定的，并且在制备后12个多月仍在使用。当应用于45份恶性贫血病例的血清样本时，30份（67%）显示出内因子抗体。该方法简单、容量大、成本低、标记物稳定，再加上相对较高的临床敏感性，使其适用于对大量样本进行经济有效的筛查。对基本检测试剂进行简单修改即可区分I型和II型内因子抗体。
