Waters H M, Dawson D W, Howarth J E, Geary C G
University Department of Clinical Haematology, Manchester Royal Infirmary.
J Clin Pathol. 1993 Jan;46(1):45-7. doi: 10.1136/jcp.46.1.45.
To investigate the incidence of type II autoantibodies to intrinsic factor in pernicious anaemia.
Three hundred and forty four serum samples submitted for intrinsic factor antibody (IFAB) analysis on clinical or laboratory grounds were tested by an established radioassay and a new enzyme linked immunosorbent assay (ELISA) method for type I and total IFAB, respectively. Sixty of these were found to be positive by ELISA; this method was used to test further, 40 samples of adequate volume for types I and II antibodies.
Type II antibodies were detected in 39 of the 40 sera tested. A comparative analysis indicated that seven samples contained pure type II antibody, being positive for total and type II by ELISA, but negative for type I by both the ELISA and radioassay technique.
The occurrence of type II antibody, both alone and in combination with type I, seems to be more common than has previously been recognised, and emphasises the advantage of using a technique which will detect both types of antibody.
研究恶性贫血中内因子II型自身抗体的发生率。
对344份因临床或实验室原因提交进行内因子抗体（IFAB）分析的血清样本，分别采用既定的放射免疫分析法和新的酶联免疫吸附测定法（ELISA）检测I型和总IFAB。其中60份经ELISA检测为阳性；该方法用于进一步检测40份有足够量的I型和II型抗体样本。
在检测的40份血清中，39份检测到II型抗体。对比分析表明，7份样本含有纯II型抗体，ELISA检测总抗体和II型抗体呈阳性，但ELISA和放射免疫分析技术检测I型抗体均为阴性。
II型抗体单独出现或与I型抗体同时出现的情况似乎比之前认为的更为常见，这强调了使用能检测两种类型抗体的技术的优势。
