Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, CP 83000 Hermosillo, Sonora, Mexico.
Departamento de Ciencias Químico Biológicas, Universidad de Sonora, CP 83000 Hermosillo, Sonora, Mexico.
Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:650-6. doi: 10.1016/j.msec.2016.03.042. Epub 2016 Mar 16.
Polypyrrole (PPy) was synthesized by enzyme mediated oxidation of pyrrole using naturally occurring compounds as redox mediators. The catalytic mechanism is an enzymatic cascade reaction in which hydrogen peroxide is the oxidizer and soybean peroxidase, in the presence of acetosyringone, syringaldehyde or vanillin, acts as a natural catalysts. The effect of the initial reaction composition on the polymerization yield and electrical conductivity of PPy was analyzed. Morphology of the PPy particles was studied by scanning electron microscopy and transmission electron microscopy whereas the chemical structure was studied by X-ray photoelectron and Fourier transformed infrared spectroscopic techniques. The redox mediators increased the polymerization yield without a significant modification of the electronic structure of PPy. The highest conductivity of PPy was reached when chondroitin sulfate was used simultaneously as dopant and template during pyrrole polymerization. Electroactive properties of PPy obtained from natural precursors were successfully used in the amperometric quantification of uric acid concentrations. PPy increases the amperometric sensitivity of carbon nanotube screen-printed electrodes toward uric acid detection.
聚吡咯（PPy）是通过使用天然化合物作为氧化还原介体酶促氧化吡咯合成的。催化机制是酶级联反应，其中过氧化氢是氧化剂，而在乙酰丁香酮、丁香醛或香草醛存在下，大豆过氧化物酶作为天然催化剂。分析了初始反应成分对 PPy 聚合产率和电导率的影响。通过扫描电子显微镜和透射电子显微镜研究了 PPy 颗粒的形态，而通过 X 射线光电子能谱和傅里叶变换红外光谱技术研究了化学结构。氧化还原介体在不显著改变 PPy 电子结构的情况下提高了聚合产率。当硫酸软骨素在吡咯聚合过程中同时用作掺杂剂和模板时，PPy 的电导率达到最高。从天然前体获得的 PPy 的电活性特性成功地用于尿酸浓度的安培定量。PPy 提高了碳纳米管丝网印刷电极对尿酸检测的安培灵敏度。
