Juricic Gordana, Saracevic Andrea, Kopcinovic Lara Milevoj, Bakliza Ana, Simundic Ana-Maria
Department of Laboratory Diagnostics, General Hospital Pula, Pula, Croatia.
University Department of Chemistry, University Hospital Centre "Sestre Milosrdnice", Zagreb, Croatia.
Clin Biochem. 2016 Dec;49(18):1402-1405. doi: 10.1016/j.clinbiochem.2016.03.006. Epub 2016 Apr 9.
Citrate buffer additive has been suggested to be of supreme performance in inhibiting glycolysis. However, there is little evidence in the literature regarding the comparability of glucose concentrations in liquid and lyophilized citrate buffer containing tubes. The aim of this study was to compare glucose concentrations in tubes containing liquid (Glucomedics) and lyophilized citrate buffer (Terumo VENOSAFE™ Glycemia) additive, measured immediately after centrifugation.
Blood was collected from forty volunteers into both Glucomedics and Venosafe Glycemia tubes. Blood was centrifuged within 15min from venipuncture and glucose concentration was measured immediately after centrifugation, on the Abbott Architect analyzer. Differences between glucose concentrations in Glucomedics and Terumo tubes were tested using the paired t-test. Mean bias was calculated and compared to recommended quality specification for glucose (i.e. 2.2%).
Glucose concentration in Terumo tubes was 3.4% lower than in Glucomedics tubes (P<0.001). The mean bias was clinically significant.
There is a clinically significant difference between glucose concentrations in liquid and lyophilized citrate buffer additive tubes (Glucomedics vs. Terumo tubes) measured immediately after centrifugation. This difference may affect the patient outcome due to the misclassification of diabetes.
有人提出柠檬酸盐缓冲添加剂在抑制糖酵解方面具有卓越性能。然而，关于含液体和冻干柠檬酸盐缓冲液试管中葡萄糖浓度的可比性，文献中几乎没有相关证据。本研究的目的是比较离心后立即测量的含液体（Glucomedics）和冻干柠檬酸盐缓冲液（Terumo VENOSAFE™血糖）添加剂试管中的葡萄糖浓度。
从40名志愿者采集血液，分别注入Glucomedics和Venosafe血糖试管。采血后15分钟内进行离心，离心后立即在雅培Architect分析仪上测量葡萄糖浓度。使用配对t检验检测Glucomedics和Terumo试管中葡萄糖浓度的差异。计算平均偏差并与葡萄糖推荐质量规范（即2.2%）进行比较。
Terumo试管中的葡萄糖浓度比Glucomedics试管中的低3.4%（P<0.001）。平均偏差具有临床显著性。
离心后立即测量时，含液体和冻干柠檬酸盐缓冲添加剂试管（Glucomedics与Terumo试管）中的葡萄糖浓度存在临床显著差异。由于糖尿病的错误分类，这种差异可能会影响患者的治疗结果。
