不同抗凝剂存在下血浆中葡萄糖的稳定性

Stability of glucose in plasma with different anticoagulants.

作者信息

Fobker Manfred

出版信息

Clin Chem Lab Med. 2014 Jul;52(7):1057-60. doi: 10.1515/cclm-2013-1049.

DOI:10.1515/cclm-2013-1049

PMID:24633752

Abstract

BACKGROUND

People with hyperglycemia, especially those who are pregnant, depend on accurate blood glucose measurements for correct diagnosis of diabetes and monitoring. Glycolysis after blood draw, however, decreases glucose concentrations in blood that is collected at room temperature in the absence of a stabilizer. Cold temperatures (4°C) inhibit glycolysis; but prompt cooling and processing of each blood sample in the cold is difficult to achieve in routine clinical practice. Therefore, preservatives are used to stabilize glucose during blood collection and processing procedures that are performed at room temperature. This study examined the effect of different anticoagulants (EDTA, heparin, oxalate), with or without glycolysis inhibitors (NaF, citrate), on the stability of glucose in plasma samples - obtained from blood that was collected and stored at room temperature for up to 24 h.

METHODS

Venous blood was collected from 60 volunteers; each donor blood sample was divided into six tubes, each one containing a different anti-glycolysis-anticoagulant composition. Terumo VENOSAFE™ Glycemia tubes contained NaF/citrate buffer)/Na2EDTA; NaF/Na-heparin; and NaF/K2oxalate. Sarstedt tubes contained NaF/citrate; NaF/Na2EDTA; and K2EDTA. At 0, 2, 8 and 24 h, plasma was obtained for glucose measurements using the Glucose Hexokinase and Glucose Oxidase methods, and the ADVIA® 1800 Clinical Chemistry System.

RESULTS

Both methods demonstrated minimal glycolysis by 24 h (<3.8%) for the three Terumo VENOSAFE™ Glycemia tubes, and the Sarstedt S-Monovette GlucoEXACT tube that contained NaF/citrate. Glycolysis was higher in tubes containing NaF/Na2EDTA-alone (11.7%) and K2EDTA-alone (85%).

CONCLUSIONS

Terumo VENOSAFE™ Glycemia tubes (containing NaF/citrate buffer/Na2EDTA; NaF/Na-heparin; and NaF/K2oxalate) and the Sarstedt S-Monovette® GlucoEXACT tubes (containing NaF/citrate) are suitable for shipping venous whole blood samples to the testing laboratory within 24 h at room temperature.

摘要

背景

高血糖患者，尤其是孕妇，依赖准确的血糖测量来正确诊断糖尿病并进行监测。然而，采血后的糖酵解会降低在室温下且无稳定剂时采集的血液中的葡萄糖浓度。低温（4°C）可抑制糖酵解；但在常规临床实践中，很难对每个血样及时进行冷却并在低温下处理。因此，在室温下进行的血液采集和处理过程中，会使用防腐剂来稳定葡萄糖。本研究考察了不同抗凝剂（乙二胺四乙酸（EDTA）、肝素、草酸盐），无论有无糖酵解抑制剂（氟化钠（NaF）、柠檬酸盐），对从采集并在室温下储存长达24小时的血液中获得的血浆样本中葡萄糖稳定性的影响。

方法

从60名志愿者采集静脉血；每个供血者的血样被分成六管，每管含有不同的抗糖酵解 - 抗凝剂组合物。泰尔茂VENOSAFE™血糖管含有NaF/柠檬酸盐缓冲液/Na2EDTA；NaF/钠肝素；以及NaF/K2草酸盐。赛多利斯管含有NaF/柠檬酸盐；NaF/Na2EDTA；以及K2EDTA。在0、2、8和24小时，使用葡萄糖己糖激酶法和葡萄糖氧化酶法以及ADVIA® 1800临床化学系统获取血浆进行葡萄糖测量。