Masson Jerome, Liberto Erica, Beolor Jean-Claude, Brevard Hugues, Bicchi Carlo, Rubiolo Patrizia
Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P. Giuria 9, I-10125 Torino, Italy; Robertet SA, Research Division, 37 Avenue Sidi Brahim, F-06130 Grasse, France.
Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P. Giuria 9, I-10125 Torino, Italy.
Food Chem. 2016 Sep 1;206:223-33. doi: 10.1016/j.foodchem.2016.03.057. Epub 2016 Mar 17.
This study aimed to characterise and discriminate 44 authenticated commercial samples of citrus essential oils (EO) from seven species (bergamot, lemon, bigarade, orange, mandarin, grapefruit, lime) by analysing the non-volatile oxygenated heterocyclic compounds (OHC) by UHPLC/TOF-HRMS, multivariate data analysis (PCA, PLS-DA) and metabolomic strategies; the OHC fraction includes coumarins, furocoumarins, and polymethoxylated flavonoids. Two different approaches were adopted: (i) targeted profiling based on quantifying 18 furocoumarins and coumarins, some of which are regulated by law, and (ii) targeted fingerprinting based on 140 OHCs reported in citrus essential oils, from which 38 discriminant markers were defined. This approach correctly discriminated the Citrus species; its "sensitivity" to relatively low adulteration rate (10%) was highly satisfactory. The proposed method is complementary to that of analysing the citrus EO volatile part by GC techniques.
本研究旨在通过超高效液相色谱/飞行时间高分辨质谱（UHPLC/TOF-HRMS）分析非挥发性氧化杂环化合物（OHC）、多变量数据分析（PCA、PLS-DA）和代谢组学策略，对来自七个品种（佛手柑、柠檬、酸橙、橙子、橘子、葡萄柚、青柠）的44个经认证的柑橘属植物精油（EO）商业样品进行表征和鉴别；OHC部分包括香豆素、呋喃香豆素和多甲氧基黄酮。采用了两种不同的方法：（i）基于对18种呋喃香豆素和香豆素进行定量的靶向分析，其中一些受法律管制；（ii）基于柑橘属植物精油中报告的140种OHC的靶向指纹图谱分析，从中定义了38个判别标记。该方法正确鉴别了柑橘属品种；其对相对较低掺假率（10%）的“灵敏度”非常令人满意。所提出的方法是对通过气相色谱技术分析柑橘属植物精油挥发性部分的方法的补充。
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