Coleman R A, Nials A T
Department of Biochemistry and Respiratory Pharmacology, Glaxo Group Research, Ware, Herts, U.K.
J Pharmacol Methods. 1989 Mar;21(1):71-86. doi: 10.1016/0160-5402(89)90023-5.
We have developed a novel, eight-chamber superfusion system that is suitable for a variety of applications involving the study of both contraction and relaxation of smooth muscle preparations, and the effect of agents that interfere with these actions. The system allows electrical stimulation of preparations, and thus neuronally mediated responses and agents that interfere with neurotransmission may also be studied. To demonstrate some of the applications of the system, we have evaluated both spasmogenic and spasmolytic agents on the guinea-pig isolated tracheal strip preparation. The potency and the times for onset and offset of action of the spasmogens, acetylcholine, histamine, and prostaglandin F2 alpha, and the spasmolytics, isoprenaline, clenbuterol, salbutamol, papaverine, N-ethylcarboxamide adenosine, theophylline, and verapamil, have been investigated. The spasmolytic agents have been tested against both prostaglandin F2 alpha-induced tone and electrically induced contractile responses of the guinea-pig trachea. This superfusion system has several advantages over previously described superfusion or immersion techniques. It is compact and allows simultaneous study of up to eight preparations. It is suitable for a wide range of tissues, and the use of this system avoids the necessity of repeatedly washing drugs from organ baths. However, one of the most important applications of the system is its use in the study of rates of onset and offset of drug action. We believe, therefore, that this system represents an important alternative to the classical organ bath for in vitro pharmacological experimentation.
我们开发了一种新型的八室灌流系统，该系统适用于多种应用，包括平滑肌制剂收缩和舒张的研究，以及干扰这些作用的药物的效应研究。该系统允许对制剂进行电刺激，因此也可以研究神经介导的反应以及干扰神经传递的药物。为了证明该系统的一些应用，我们评估了致痉剂和解痉剂对豚鼠离体气管条制剂的作用。研究了致痉剂乙酰胆碱、组胺和前列腺素F2α以及解痉剂异丙肾上腺素、克伦特罗、沙丁胺醇、罂粟碱、N - 乙基羧酰胺腺苷、茶碱和维拉帕米的效力、起效时间和作用消退时间。已针对前列腺素F2α诱导的张力以及豚鼠气管的电诱导收缩反应测试了解痉剂。与先前描述的灌流或浸泡技术相比，该灌流系统具有几个优点。它结构紧凑，允许同时研究多达八个制剂。它适用于广泛的组织，并且使用该系统避免了从器官浴中反复冲洗药物的必要性。然而，该系统最重要的应用之一是其在药物作用起效和消退速率研究中的应用。因此，我们认为该系统是体外药理实验中经典器官浴的重要替代方法。
