Roseland Janet Maxwell, Patterson Kristine Y, Andrews Karen W, Phillips Katherine M, Phillips Melissa M, Pehrsson Pamela R, Dufresne Guy L, Jakobsen Jette, Gusev Pavel A, Savarala Sushma, Nguyen Quynhanh V, Makowski Andrew J, Scheuerell Chad R, Larouche Guillaume P, Wise Stephen A, Harnly James M, Williams Juhi R, Betz Joseph M, Taylor Christine L
Nutrient Data Laboratory, Agricultural Research Service, U.S. Department of Agriculture , 10300 Baltimore Avenue, Building 005, BARC-West, Beltsville, Maryland 20705, United States.
Biochemistry Department (0308), Virginia Tech , 304 Engel Hall, Blacksburg, Virginia 24061, United States.
J Agric Food Chem. 2016 Apr 27;64(16):3167-75. doi: 10.1021/acs.jafc.5b05016. Epub 2016 Apr 15.
Assessment of total vitamin D intake from foods and dietary supplements (DSs) may be incomplete if 25-hydroxyvitamin D [25(OH)D] intake is not included. However, 25(OH)D data for such intake assessments are lacking, no food or DS reference materials (RMs) are available, and comparison of laboratory performance has been needed. The primary goal of this study was to evaluate whether vitamin D3 and 25(OH)D3 concentrations in food and DS materials could be measured with acceptable reproducibility. Five experienced laboratories from the United States and other countries participated, all using liquid chromatography tandem-mass spectrometry but no common analytical protocol; however, various methods were used for determining vitamin D3 in the DS. Five animal-based materials (including three commercially available RMs) and one DS were analyzed. Reproducibility results for the materials were acceptable. Thus, it is possible to obtain consistent results among experienced laboratories for vitamin D3 and 25(OH)D3 in foods and a DS.
如果不纳入25-羟基维生素D[25(OH)D]的摄入量，那么对食物和膳食补充剂（DSs）中总维生素D摄入量的评估可能是不完整的。然而，此类摄入量评估缺乏25(OH)D数据，没有可用的食物或DS参考物质（RMs），并且需要对实验室性能进行比较。本研究的主要目标是评估食物和DS材料中维生素D3和25(OH)D3的浓度是否能够以可接受的重现性进行测量。来自美国和其他国家的五个经验丰富的实验室参与其中，所有实验室都使用液相色谱串联质谱法，但没有共同的分析方案；然而，测定DS中维生素D3使用了各种方法。分析了五种基于动物的材料（包括三种市售RMs）和一种DS。这些材料的重现性结果是可接受的。因此，经验丰富的实验室对于食物和一种DS中的维生素D3和25(OH)D3能够获得一致的结果。
