竹叶提取物溶液和叶绿素铜钠对流感病毒和人类免疫缺陷病毒细胞病变效应的体外抑制作用
In Vitro Inhibition of Cytopathic Effect of Influenza Virus and Human Immunodeficiency Virus by Bamboo Leaf Extract Solution and Sodium Copper Chlorophyllin.
作者信息
Ito Akiko, Tsuneki Akeno, Yoshida Yu, Ryoke Kazuo, Kaidoh Toshiyuki, Kageyama Seiji
机构信息
Division of Oral and Maxillofacial Biopathological Surgery, Department of Medicine of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.
†Division of Virology, Department of Microbiology and Immunology, School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8503, Japan.
出版信息
Yonago Acta Med. 2016 Mar;59(1):61-5. Epub 2016 Apr 1.
BACKGROUND
Although the link between oral and oropharyngeal health status and susceptibility to infection has long been recognized, there is a limit to the selection of antiseptics for oral care.
METHODS
Madin-Darby canine kidney (MDCK) cells were exposed to influenza virus and cultured in the presence or absence of test reagents: bamboo leaf extract solution and sodium copper chrolophyllin. MDCK cells were pre-incubated with the reagents to assess the inhibitory activity at adsorption (viral attachment). Similarly, anti-HIV activity and the inhibitory mechanism at adsorption were assessed by MT-2 cell culture system. Mixture of HIV and bamboo leaf extract solution was fixed and examined by transmission electron microscopy.
RESULTS
The 50% inhibitory concentration (IC50) of bamboo leaf extract solution against influenza virus and the 50% cytotoxic concentration (CC50) in MDCK cells of the solution lay between 0.0313-0.0625% and 0.5-1.0%. The solution inhibited the influenza virus adsorption at the concentration of 0.5% (P < 0.05). The values of IC50 and CC50 of sodium copper chlorophyllin lay between 50-100 µM and 200-400 µM, respectively. This inhibited the virus adsorption at 200 µM (P < 0.05). The bamboo leaf extract solution showed values of IC50 against HIV and CC50 in MT-2 cells at around 0.0313% and between 0.25-0.5%, respectively. This solution inhibited HIV adsorption at 1.25% (P < 0.05). The IC50 and CC50 of sodium copper chlorophyllin lay between 50-100 µM and 200-400 µM, respectively. Sodium copper chlorophyllin inhibited HIV adsorption at 2.5 mM (P < 0.05). HIV particles survived after the exposure to 0.5% bamboo leaf extract solution.
CONCLUSION
Sodium copper chlorophyllin exerted antiviral activities against influenza virus and HIV as the major ingredient of bamboo leaf extract solution by blocking adsorption. This mechanism of action is different completely from the one of povidone-iodine.
背景
尽管口腔和口咽健康状况与感染易感性之间的联系早已为人所知，但口腔护理用防腐剂的选择有限。
方法
将Madin-Darby犬肾（MDCK）细胞暴露于流感病毒，并在有或无测试试剂（竹叶提取物溶液和叶绿素铜钠）的情况下进行培养。将MDCK细胞与试剂预孵育，以评估在吸附（病毒附着）时的抑制活性。同样，通过MT-2细胞培养系统评估抗HIV活性及吸附时的抑制机制。将HIV与竹叶提取物溶液的混合物固定后，用透射电子显微镜检查。
结果
竹叶提取物溶液对流感病毒的50%抑制浓度（IC50）及在MDCK细胞中的50%细胞毒性浓度（CC50）分别在0.0313 - 0.0625%和0.5 - 1.0%之间。该溶液在浓度为0.5%时抑制流感病毒吸附（P < 0.05）。叶绿素铜钠的IC50和CC50值分别在50 - 100 μM和200 - 400 μM之间。其在200 μM时抑制病毒吸附（P < 0.05）。竹叶提取物溶液在MT-2细胞中对HIV的IC50值和约CC50值分别在0.0313%左右和0.25 - 0.5%之间。该溶液在1.25%时抑制HIV吸附（P < 0.05）。叶绿素铜钠的IC50和CC50分别在50 - 100 μM和200 - 400 μM之间。叶绿素铜钠在2.5 mM时抑制HIV吸附（P < 0.05）。暴露于0.5%竹叶提取物溶液后HIV颗粒存活。
结论
叶绿素铜钠作为竹叶提取物溶液的主要成分，通过阻断吸附对流感病毒和HIV发挥抗病毒活性。这种作用机制与聚维酮碘的作用机制完全不同。
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