Wedler Jonas, Daubitz Tony, Schlotterbeck Götz, Butterweck Veronika
Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland.
Institute for Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland.
Planta Med. 2014 Dec;80(18):1678-84. doi: 10.1055/s-0034-1383195. Epub 2014 Oct 15.
Extracts prepared from the leaves of Phyllostachys edulis (bamboo) have received attention in pharmacological research due to their potent antitumor, anti-inflammatory, antimicrobial, and anti-ulcerogenic activities. In this study, anti-inflammatory effects of a bamboo leaf extract on tumor necrosis factor alpha-induced overproduction of interleukin 8, vascular endothelial growth factor, and interleukin 6 in immortalized human keratinocytes were investigated for the first time. In addition, wound-healing effects were evaluated in 3T3-swiss albino mouse fibroblasts. Bamboo leaf extract and isoorientin inhibited the tumor necrosis factor alpha-induced release of interleukin 8 and vascular endothelial growth factor. Furthermore, isoorientin dose-dependently reduced levels of interleukin 6 in tumor necrosis factor alpha-α-treated immortalized human keratinocytes cells. Wound healing was evaluated using a modification of the classical scratch assay. For evaluation of the wound gap, a new computerized method based on time-lapse microscopy was developed. It was shown that bamboo leaf extract (10 µg/mL) improved wound closure by 28 % (12 h) and 54 % (24 h), respectively. In concentrations of 50 µg/mL and above, bamboo leaf extract inhibited cell migration without affecting cell viability. Isoorientin (10 µM) improved wound closure by 29 % (12 h) and 56 % (24 h), respectively. Comparable to bamboo leaf extract, higher concentrations of isoorientin prevented cell migration. It is suggested that bamboo leaf extract as well as isoorientin have a dual activity - in higher doses, they show anti-inflammatory effects, and in lower concentrations, they exert anti-angiogenic activities.
毛竹竹叶提取物因其具有强大的抗肿瘤、抗炎、抗菌和抗溃疡活性而在药理学研究中受到关注。在本研究中，首次研究了竹叶提取物对肿瘤坏死因子α诱导的永生化人角质形成细胞中白细胞介素8、血管内皮生长因子和白细胞介素6过量产生的抗炎作用。此外，还在3T3-瑞士白化小鼠成纤维细胞中评估了其伤口愈合作用。竹叶提取物和异荭草素抑制了肿瘤坏死因子α诱导的白细胞介素8和血管内皮生长因子的释放。此外，异荭草素剂量依赖性地降低了肿瘤坏死因子α处理的永生化人角质形成细胞中白细胞介素6的水平。使用改良的经典划痕试验评估伤口愈合情况。为了评估伤口间隙，开发了一种基于延时显微镜的新计算机化方法。结果表明，竹叶提取物（10μg/mL）分别使伤口闭合率提高了28%（12小时）和54%（24小时）。在浓度为50μg/mL及以上时，竹叶提取物抑制细胞迁移但不影响细胞活力。异荭草素（10μM）分别使伤口闭合率提高了29%（12小时）和56%（24小时）。与竹叶提取物类似，较高浓度的异荭草素可阻止细胞迁移。提示竹叶提取物和异荭草素具有双重活性——高剂量时表现出抗炎作用，低浓度时发挥抗血管生成活性。
