Zhu L L, Wang C H, Yang H P, Shu W H
Department of Orthopaedics, the Peoples Hospital of Liaocheng City, Shandong, China.
Medical Basic Teaching and Research Office, School of Nursing, Binzhou Polytechnic, Shandong, China.
J Biol Regul Homeost Agents. 2016 Jan-Mar;30(1):103-10.
This study was carried out to investigate the expression changes of RanBP9 in tissue specimens and osteosarcoma cell strains and preliminarily explore its mechanism in osteosarcoma, so as to provide a theoretical foundation for follow-up experiments. The expression of RanBP9 in human osteosarcoma tissue specimens was detected by immunohistochemistry and the expression of RanBP9 messenger ribose nucleic acid (mRNA) in osteosarcoma cell strains was detected in real-time with polymerase chain reaction (PCR), and finally the expression of RanBP9 protein in osteosarcoma cell strains was detected by immunofluorescent staining and Western blot. Results demonstrated that RanBP9 was widely expressed in tissues, but also highly expressed in cells; moreover, the expression of RanBP9 was mainly concentrated in cytoplasm and nucleus, and partial expression was found in cell membrane. Thus, it can be concluded that RanBP9 is positively expressed in bone tumor tissues and cell strains.
本研究旨在探讨RanBP9在组织标本和骨肉瘤细胞株中的表达变化，并初步探究其在骨肉瘤中的作用机制，为后续实验提供理论依据。采用免疫组织化学法检测人骨肉瘤组织标本中RanBP9的表达，应用聚合酶链反应（PCR）实时检测骨肉瘤细胞株中RanBP9信使核糖核酸（mRNA）的表达，最后通过免疫荧光染色和蛋白质免疫印迹法检测骨肉瘤细胞株中RanBP9蛋白的表达。结果显示，RanBP9不仅在组织中广泛表达，在细胞中也高表达；而且，RanBP9的表达主要集中在细胞质和细胞核，部分表达于细胞膜。因此，可以得出结论，RanBP9在骨肿瘤组织和细胞株中呈阳性表达。
