Januszko Olga, Madej Dawid, Brzozowska Anna, Kaluza Joanna
Department of Human Nutrition, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.
Department of Human Nutrition, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.
J Trace Elem Med Biol. 2016 May;35:77-82. doi: 10.1016/j.jtemb.2016.01.013. Epub 2016 Jan 30.
The aim of this study was to investigate the changes in iron apparent absorption (IAA%) during and after iron and zinc supplementation in rats. The study was conducted on 6-week old male Wistar rats in 3 stages: 4-week period of adaptation to the control (C) and iron deficient (D) diets (stage I); 4-week period of supplementation with 10-time more iron (CSFe, DSFe), zinc (CSZn, DSZn) or both iron and zinc (CSFeZn, DSFeZn) compared to C diet (stage II); 2-week of post-supplementation period (rats were fed the same diets as in the adaptation period, stage III). IAA% was measured in five consecutive days directly after introducing and discontinuation of iron and zinc supplementation as well as in the end of stage II (days: 22-24th) and stage III (days: 8-10th). Overall in the second day after introducing and in the fifth day after discontinuation of iron or iron and zinc supplementation, the IAA% had undergone to the level compatible with the values in the end of each stage. At the end of stage II, IAA% in CSFeZn (54.1 ± 2.7%) rats was not different from the IAA% in CSFe rats (53.9 ± 1.9%), but in DSFeZn group IAA% (49.4 ± 2.1%) was significantly lower than in DSFe (57.4 ± 2.3%) group. Moreover, IAA% after stage II and stage III in DSZn group was significantly lower (39.2 ± 2.8% and 38.6 ± 2.6%, respectively) than in group D (60.7 ± 1.9% and 54.3 ± 3.0%, respectively). In conclusion, zinc administered simultaneously with iron (Zn:Fe weight ratio=1:1) decreased IAA% in adult rats fed on iron deficient diet, but not in rats fed on control diet. IAA% reduction by zinc supplementation has been extended to 10 days after discontinuation of the treatment. Adaptation of the rats to high doses of iron or iron and zinc and also to the cessation of these treatments was relatively fast. However, IAA% was stabilized faster after introducing the supplementation than it's discontinuation.
本研究的目的是调查大鼠补充铁和锌期间及之后铁的表观吸收率（IAA%）的变化。该研究以6周龄雄性Wistar大鼠为对象，分三个阶段进行：4周适应对照（C）和缺铁（D）饮食的时期（第一阶段）；与C饮食相比，补充10倍剂量铁（CSFe、DSFe）、锌（CSZn、DSZn）或铁和锌两者（CSFeZn、DSFeZn）的4周时期（第二阶段）；补充后2周时期（大鼠喂食与适应期相同的饮食，第三阶段）。在引入和停止补充铁和锌后连续五天以及在第二阶段末（第22 - 24天）和第三阶段末（第8 - 10天）测量IAA%。总体而言，在引入铁或铁和锌补充剂后的第二天以及停止补充后的第五天，IAA%已达到与每个阶段末的值相符的水平。在第二阶段末，CSFeZn组（54.1±2.7%）大鼠的IAA%与CSFe组（53.9±1.9%）大鼠的IAA%无差异，但在DSFeZn组中，IAA%（49.4±2.1%）显著低于DSFe组（57.4±2.3%）。此外，DSZn组在第二阶段和第三阶段后的IAA%（分别为39.2±2.8%和38.6±2.6%）显著低于D组（分别为60.7±1.9%和54.3±3.0%）。总之，与铁同时给予锌（锌：铁重量比 = 1:1）会降低缺铁饮食成年大鼠的IAA%，但对喂食对照饮食的大鼠无此影响。补充锌导致的IAA%降低在停止治疗后持续了10天。大鼠对高剂量铁或铁和锌以及停止这些治疗的适应相对较快。然而，引入补充剂后IAA%比停止补充剂后更快稳定。
