Broeckx Géraldine, Vandenheuvel Dieter, Claes Ingmar J J, Lebeer Sarah, Kiekens Filip
University of Antwerp, Department of Pharmaceutical, Biomedical and Veterinary Sciences, Laboratory of Pharmaceutical Technology and Biopharmacy, Universiteitsplein 1, B-2610 Wilrijk, Belgium; University of Antwerp, Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
University of Antwerp, Department of Bioscience Engineering, Research Group Environmental Ecology and Applied Microbiology, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
Int J Pharm. 2016 May 30;505(1-2):303-18. doi: 10.1016/j.ijpharm.2016.04.002. Epub 2016 Apr 2.
The increasing knowledge about the human microbiome leads to the awareness of how important probiotics can be for our health. Although further substantiation is required, it appears that several pathologies could be treated or prevented by the administration of pharmaceutical formulations containing such live health-beneficial bacteria. These pharmabiotics need to provide their effects until the end of shelf life, which can be optimally achieved by drying them before further formulation. However, drying processes, including spray-, freeze-, vacuum- and fluidized bed drying, induce stress on probiotics, thus decreasing their viability. Several protection strategies can be envisaged to enhance their viability, including addition of protective agents, controlling the process parameters and prestressing the probiotics prior to drying. Moreover, probiotic viability needs to be maintained during long-term storage. Overall, lower storage temperature and low moisture content result in good survival rates. Attention should also be given to the rehydration conditions of the dried probiotics, as this can exert an important effect on their revival. By describing not only the characteristics, but also the viability results obtained by the most relevant drying techniques in the probiotic industry, we hope to facilitate the deliberate choice of drying process and protection strategy for specific probiotic and pharmabiotic applications.
对人类微生物组的了解日益增加，使人们意识到益生菌对我们的健康有多重要。尽管还需要进一步证实，但似乎通过服用含有此类有益健康的活细菌的药物制剂，可以治疗或预防几种疾病。这些药物益生菌需要在保质期结束前发挥其作用，这可以通过在进一步制剂之前将它们干燥来最佳地实现。然而，包括喷雾干燥、冷冻干燥、真空干燥和流化床干燥在内的干燥过程会给益生菌带来压力，从而降低它们的活力。可以设想几种保护策略来提高它们的活力，包括添加保护剂、控制工艺参数以及在干燥前对益生菌进行预应激处理。此外，在长期储存期间需要保持益生菌的活力。总体而言，较低的储存温度和低水分含量可带来良好的存活率。还应注意干燥益生菌的复水条件，因为这会对它们的复苏产生重要影响。通过不仅描述最相关的干燥技术在益生菌行业中获得的特性，还描述其活力结果，我们希望为特定的益生菌和药物益生菌应用促进干燥工艺和保护策略的审慎选择。
