利用454焦磷酸测序技术开发银杏新型微卫星标记并进行特征分析。

Development and characterization of novel microsatellite markers for Ginkgo biloba using 454 pyrosequencing.

作者信息

Zhou Q, Wang X X, Xu M, Cao F L, Yu F X, Xu L A

机构信息

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.

Jiangxi Provincial Key Laboratory of Ornamental Plant Genetic Improvement, Research Institute for Bioresources, Jiangxi Academy of Sciences, Nanchang, China.

出版信息

Genet Mol Res. 2016 Mar 28;15(1):gmr7385. doi: 10.4238/gmr.15017385.

DOI:10.4238/gmr.15017385

PMID:27051011

Abstract

As a "living fossil" that is used to understand the evolutionary history of seed plants, Ginkgo biloba is a well-known multipurpose tree with edible seeds, medicinal properties, and ornamental value, but little is known about its genetic diversity. Microsatellite, or simple sequence repeat (SSR), markers have proven to be powerful tools for genetic studies of plants. In this study, we isolated 30 novel polymorphic microsatellite loci in G. biloba using 454 pyrosequencing. The characteristics of these loci were tested with 48 cultivars. The number of alleles (NA) per locus ranged from two to seven. The observed (HO) and expected (HE) heterozygosities ranged from 0.000 to 0.750 and from 0.021 to 0.792, with an average of 0.326 and 0.443, respectively. In terms of genetic diversity in the Ginkgo population, NA was 3.300, NE was 2.090, I was 0.782, HO was 0.326, and HE was 0.443. These polymorphic SSRs will be useful for the assessment of population genetic diversity and resource conservation of G. biloba.

摘要

作为一种用于了解种子植物进化历史的“活化石”，银杏是一种著名的多功能树种，其种子可食用，具有药用价值和观赏价值，但人们对其遗传多样性知之甚少。微卫星，即简单序列重复（SSR）标记，已被证明是植物遗传研究的有力工具。在本研究中，我们利用454焦磷酸测序技术在银杏中分离出30个新的多态性微卫星位点。用48个品种对这些位点的特征进行了测试。每个位点的等位基因数（NA）范围为2至7个。观察到的杂合度（HO）和预期杂合度（HE）范围分别为0.000至0.750和0.021至0.792，平均分别为0.326和0.443。就银杏种群的遗传多样性而言，NA为3.300，NE为2.090，I为0.782，HO为0.326，HE为0.443。这些多态性SSR将有助于评估银杏的种群遗传多样性和资源保护。