红鳍笛鲷新型多态微卫星标记的分离与特征分析
Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus.
作者信息
Lin L, Li C H, Xu S N, Liu Y, Xiao Y Y
机构信息
Key Laboratory for Exploitation & Utilization of Marine Fisheries Resources in the South China Sea, Ministry of Agriculture, Guangzhou, China.
出版信息
Genet Mol Res. 2015 Sep 21;14(3):10944-7. doi: 10.4238/2015.September.21.5.
We isolated and characterized 22 polymorphic microsatellite loci in the crimson snapper (Lutjanus erythropterus) using a (GT)13-enriched genomic library. We found three to 15 alleles per locus, with a mean of 6.68. The observed and expected heterozygosities ranged from 0.087 to 0.978 and from 0.125 to 0.904, respectively, with averages of 0.576 and 0.650, respectively. Only three loci showed significant deviation from the Hardy-Weinberg equilibrium after Bonferroni correction. Four loci showed evidence for null alleles. These markers will be useful for analyzing the population genetic structure and gene flow of L. erythropterus.
我们利用一个富含(GT)13的基因组文库，在紫红笛鲷（Lutjanus erythropterus）中分离并鉴定了22个多态微卫星位点。我们发现每个位点有3至15个等位基因，平均为6.68个。观察到的杂合度和期望杂合度分别在0.087至0.978和0.125至0.904之间，平均值分别为0.576和0.650。经Bonferroni校正后，只有三个位点显著偏离哈迪-温伯格平衡。四个位点显示存在无效等位基因的证据。这些标记物将有助于分析紫红笛鲷的种群遗传结构和基因流动。
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