嗜内脏速发型新城疫病毒在感染鸡羽毛中的复制

Viscerotropic velogenic Newcastle disease virus replication in feathers of infected chickens.

作者信息

Lee Dong-Hun, Kwon Jung-Hoon, Noh Jin-Yong, Park Jae-Keun, Yuk Seong-Su, Erdene-Ochir Tseren-Ochir, Nahm Sang-Soep, Kwon Yong-Kuk, Lee Sang-Won, Song Chang-Seon

机构信息

Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.

Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.

出版信息

J Vet Sci. 2016 Mar;17(1):115-7. doi: 10.4142/jvs.2016.17.1.115. Epub 2016 Mar 22.

DOI:10.4142/jvs.2016.17.1.115

PMID:27051348

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4808636/

Abstract

Newcastle disease viruses (NDVs) cause systemic diseases in chickens with high mortality. However, little is known about persistence of NDVs in contaminated tissues from infected birds. In this study, we examined viral replication in the feather pulp of chickens inoculated with viscerotropic velogenic NDV (vvNDV) genotype VII. Reverse transcription real-time PCR and immunohistochemistry were used to investigate viral persistence in the samples. vvNDV was detected in the oropharynx and cloaca and viral antigens were detected in the feathers, suggesting that feathers act as sources of viral transmission.

摘要

新城疫病毒（NDV）可导致鸡群发生全身性疾病，死亡率很高。然而，关于NDV在受感染禽类污染组织中的持续性知之甚少。在本研究中，我们检测了接种嗜内脏速发型新城疫病毒（vvNDV）基因型VII的鸡羽毛髓中的病毒复制情况。采用逆转录实时PCR和免疫组织化学方法研究样本中的病毒持续性。在口咽和泄殖腔中检测到vvNDV，在羽毛中检测到病毒抗原，这表明羽毛是病毒传播的来源。