Carr J P, Beachy R N, Klessig D F
Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08854.
Virology. 1989 Apr;169(2):470-3. doi: 10.1016/0042-6822(89)90175-x.
Transgenic tobacco plants constitutively expressing the coat protein (CP) of tobacco mosaic virus (TMV) exhibit enhanced resistance ot TMV (P. Powell Abel, R. S. Nelson, B. De, N. Hoffman, S. G. Rogers, R. T. Fraley, and R. N. Beachy, Science, 232, 738-743, 1986; R. S. Nelson, P. Powell Abel, and R. N. Beachy, Virology 158, 128-132, 1987). To determine if this enhanced resistance might be mediated through the PR1 family of pathogenesis-related (PR) proteins, their synthesis was examined. In transgenic plants derived from NN genotypic tobacco, a high proportion (approximately 80%) of those producing CP also expressed the PR1 genes at low levels. However, this correlation between TMV CP and PR1 gene expression was not observed in similarly transformed nn genotypic tobacco plants. Therefore, it appears unlikely that PR1 proteins play a critical role in genetically engineered resistance in transgenic plants producing TMV CP.
组成型表达烟草花叶病毒（TMV）外壳蛋白（CP）的转基因烟草植株对TMV表现出增强的抗性（P. 鲍威尔·阿贝尔、R. S. 纳尔逊、B. 德、N. 霍夫曼、S. G. 罗杰斯、R. T. 弗雷利和R. N. 比奇，《科学》，232卷，738 - 743页，1986年；R. S. 纳尔逊、P. 鲍威尔·阿贝尔和R. N. 比奇，《病毒学》，158卷，128 - 132页，1987年）。为了确定这种增强的抗性是否可能通过病程相关（PR）蛋白的PR1家族介导，对它们的合成进行了检测。在源自NN基因型烟草的转基因植株中，产生CP的植株中有很大比例（约80%）也低水平表达PR1基因。然而，在类似转化的nn基因型烟草植株中未观察到TMV CP与PR1基因表达之间的这种相关性。因此，PR1蛋白似乎不太可能在产生TMV CP的转基因植株的基因工程抗性中起关键作用。
