School of Pharmacy, East China University of Science and Technology , Shanghai 200237, China.
Thermo Fisher Scientific , 445 Lakeside Drive, Sunnyvale, California 94087, United States.
Anal Chem. 2016 May 3;88(9):4676-81. doi: 10.1021/acs.analchem.5b04384. Epub 2016 Apr 15.
We report a poly(vinyl alcohol) (PVA)-coated porous graphitic carbon (PGC, Hypercarb) packing as a novel stationary phase for hydrophilic interaction liquid chromatography (HILIC). The exterior and the pores of the PGC particles are coated with a thin layer of PVA by soaking the particles in a PVA solution, filtering, and thermally cross-linking the PVA. Such PVA coated PGC particles (5.7 μm diameter), hereinafter called PVA-PGC are stable at least through pH 1.0-12.7, can be made in <2 h, and exhibit different selectivity relative to six commercial HILIC phases and bare PGC. To our knowledge, this is the first fully pH-stable, completely neutral HILIC phase. Excellent efficiency stable is observed for polar analytes (∼70 000 and 118 000 plates/m for cytosine and resorcinol, respectively). Retention closely resembles standard HILIC behavior. Other substances can also be easily incorporated in the PVA layer; an anion exchange column can be readily made by incorporating diallyldimethylammonium chloride in the PVA coating solution. The ease of preparation without the requirement of synthetic skills or paraphernalia and the possibility of incorporating a variety of modifiers makes this a particularly versatile approach.
我们报告了一种聚（乙烯醇）（PVA）涂覆的多孔石墨化碳（PGC，Hypercarb）填充剂，作为一种新型的亲水相互作用液相色谱（HILIC）固定相。通过将 PGC 颗粒浸泡在 PVA 溶液中、过滤并对 PVA 进行热交联，可以在 PGC 颗粒的外表面和孔内涂覆一层很薄的 PVA。这种经过 PVA 涂覆的 PGC 颗粒（直径 5.7μm），以下称为 PVA-PGC，至少在 pH 值 1.0-12.7 之间稳定，可以在<2 小时内制备完成，并且与六种商业 HILIC 固定相和裸 PGC 相比具有不同的选择性。据我们所知，这是第一个完全 pH 稳定、完全中性的 HILIC 固定相。极性分析物（胞嘧啶和间苯二酚的分别为约 70000 和 118000 板/米）表现出出色的高效稳定性。保留行为与标准 HILIC 行为非常相似。其他物质也可以很容易地掺入 PVA 层中；通过将二烯丙基二甲基氯化铵掺入 PVA 涂覆溶液中，可以很容易地制备阴离子交换柱。这种方法制备简单，不需要合成技能或仪器设备，并且可以掺入各种修饰剂，因此是一种特别通用的方法。
