四种海洋中心硅藻中硝酸盐、磷酸盐和硅酸盐缺乏的细胞学表现的光学显微镜观察

LIGHT MICROSCOPE OBSERVATIONS ON CYTOLOGICAL MANIFESTATIONS OF NITRATE, PHOSPHATE, AND SILICATE DEFICIENCY IN FOUR MARINE CENTRIC DIATOMS.

作者信息

Holmes R W

机构信息

Institute of Marine Resources, University of California, San Diego, La Jolla, California 92037.

出版信息

J Phycol. 1966 Dec;2(4):136-40. doi: 10.1111/j.1529-8817.1966.tb04610.x.

DOI:10.1111/j.1529-8817.1966.tb04610.x

PMID:27053480

Abstract

Cytological changes induced by nitrate or phosphate limitation were reproducible and readily visible by phase contrast microscopy in Coscinodiscus wailesii, Ditylum brightwellii, Rhizosolenia hebetata f. semi-spina, Skeletonema costatum, and Stephanopyxis turris. These effects included changes in chromatophore number and shape, pyrenoid location, and mitochondrial abundance and appearance. In S. costatum, a diatom containing only 1 chromatophore, only nitrogen-deficient cells could be recognized. Silicate deficiency prevented cell division almost entirely. When a few cells did divide in the presence of low silicate, abnormalities in valve structure occurred in some cells. Cytological differences with N and P deficiency may permit a rough assessment of the physiological condition of the same or similar species in natural diatom communities.

摘要

在威氏圆筛藻、布氏双尾藻、半棘根管藻、中肋骨条藻和塔形筛板藻中，硝酸盐或磷酸盐限制所引发的细胞学变化具有可重复性，并且通过相差显微镜很容易观察到。这些影响包括色素体数量和形状的变化、蛋白核位置、线粒体丰度和外观的变化。在仅含有1个色素体的硅藻——中肋骨条藻中，只有缺氮的细胞能够被识别。硅缺乏几乎完全阻止了细胞分裂。当少数细胞在低硅条件下进行分裂时，一些细胞的瓣膜结构出现异常。与氮和磷缺乏相关的细胞学差异可能有助于对自然硅藻群落中相同或相似物种的生理状况进行粗略评估。