Bastos Carolina R V, Maia Inês B, Pereira Hugo, Navalho João, Varela João C S
Centre of Marine Sciences, Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal.
Necton S.A., Belamandil, 8700-152 Olhão, Portugal.
Biology (Basel). 2022 Apr 14;11(4):594. doi: 10.3390/biology11040594.
and are two cosmopolitan high-value centric diatoms, with a rich nutritional profile. The following work optimised the culture medium of and cultures, respectively, in a stepwise process as follows: 2.4 mM and 1.2 mM of silicate, 4 mM of nitrate, 100 µM of phosphate, 20 and 80 µM iron, and 0.5 mL L of micronutrients. The results that were obtained revealed an increase in biomass productivity with a 1.8- and 3.2-fold increase in biomass that was produced by and , respectively. The biochemical profile showed an increase in high-value PUFAs such as 2.6-fold and 2.3-fold increase in EPA for and , respectively, whilst a 2.6-fold increase in DHA was detected in cultures. The present work provides the basic tools for the industrial cultivation of and with enhanced productivity as well as improved biomass quality, two factors which are highly relevant for a more effective application of these diatoms to aquaculture and nutraceutical production.
[具体硅藻名称1]和[具体硅藻名称2]是两种分布广泛的高价值中心硅藻，具有丰富的营养成分。以下工作分别对[具体硅藻名称1]和[具体硅藻名称2]的培养基进行了逐步优化，具体如下：2.4 mM和1.2 mM的硅酸盐、4 mM的硝酸盐、100 µM的磷酸盐、20和80 µM的铁以及0.5 mL/L的微量元素。所获得的结果表明，生物量生产力有所提高，[具体硅藻名称1]和[具体硅藻名称2]产生的生物量分别增加了1.8倍和3.2倍。生化分析表明，高价值多不饱和脂肪酸有所增加，[具体硅藻名称1]和[具体硅藻名称2]的二十碳五烯酸（EPA）分别增加了2.6倍和2.3倍，同时在[具体硅藻名称2]培养物中检测到二十二碳六烯酸（DHA）增加了2.6倍。目前的工作为[具体硅藻名称1]和[具体硅藻名称2]的工业化培养提供了基本工具，提高了生产力并改善了生物量质量，这两个因素对于更有效地将这些硅藻应用于水产养殖和营养保健品生产高度相关。
