Ibragimova S M, Trifonova E A, Filipenko E A, Shymny V K
Genetika. 2015 Dec;51(12):1368-75.
Arabidopsis thaliana delta1-pyrroline-5-carhoxylate synthase 1 gene (P5CS1) cDNA was cloned under the control of the potent constitutive 35S RNA promoter of the cauliflower mosaic virus and transferred into genome of tobacco cv. Petit Havana SR-1 (Nicotiana tabacum L.) plants. It is shown that the constitutive level of proline in the transgenic plants T0 exceeds that of the SR1 reference line by 1.5 to 4 times. Under conditions of salt stress (200, 300 mM NaCl) T1-generation transgenic plants in early stages of development formed a large biomass, developed more quickly, and had a higher rate of root growth compared to the control, which confirms the involvement of the P5CS1 gene in molecular mechanisms of stress resistance in plants.
拟南芥Δ1-吡咯啉-5-羧酸合成酶1基因（P5CS1）的cDNA在花椰菜花叶病毒强组成型35S RNA启动子的控制下被克隆，并转入烟草品种Petit Havana SR-1（烟草）的基因组中。结果表明，转基因T0代植株中脯氨酸的组成水平比SR1对照品系高出1.5至4倍。在盐胁迫条件下（200、300 mM NaCl），T1代转基因植株在发育早期形成了更大的生物量，生长更快，与对照相比根系生长速率更高，这证实了P5CS1基因参与植物抗逆的分子机制。
