Institute of Surface-Earth System Science, Tianjin University, Tianjin, 300072, China.
College of Life Sciences, Nankai University, Tianjin, 300071, China.
J Plant Res. 2019 Nov;132(6):825-837. doi: 10.1007/s10265-019-01133-7. Epub 2019 Sep 3.
Drought is an important environmental factor that can severely affect plant growth and reproduction. Although many genes related to drought tolerance have been studied in economically important crops, very few genes have been functionally identified in Malus sieversii. In this study, we isolated a new gene based on throughput RNA sequencing analysis and constructed genetic expression vectors and transformed in Arabidopsis thaliana for functional verification. The results showed that MsUspA ectopic expression driven by constitutive (CaMV 35S) promoter gave rise to substantial improvements in ability of transgenic A. thaliana plants to survive under extreme drought conditions. Improved drought resistance mainly depends on more compact cellular structure, longer roots, strong resilience and low-level ROS. Molecular expression analysis showed that MsUspA may be involved in hormone and secondary metabolite synthesis regulation to improve drought resistance.
干旱是一种重要的环境因素，会严重影响植物的生长和繁殖。尽管在经济上重要的作物中已经研究了许多与耐旱性相关的基因，但在苹果属西府海棠中，仅有极少数基因被功能鉴定。在这项研究中，我们基于高通量 RNA 测序分析分离了一个新基因，并构建了遗传表达载体，转化拟南芥进行功能验证。结果表明，由组成型启动子（CaMV35S）驱动的 MsUspA 异位表达使转基因拟南芥植物在极端干旱条件下的生存能力得到显著提高。抗旱性的提高主要取决于更紧凑的细胞结构、更长的根、更强的弹性和低水平的 ROS。分子表达分析表明，MsUspA 可能参与激素和次生代谢物合成的调节，以提高耐旱性。
