Xiong Ke, Wang Xiao-Ling, Zhi Hui-Wei, Sun Bao-Guo, Li Xiu-Ting
Beijing Laboratory for Food Quality and Safety, Beijing Technology & Business University (BTBU), No. 33 Fucheng Road, Haidian, Beijing, 100048, China.
Beijing Innovation Centre of Food Nutrition and Human, Beijing Technology & Business University (BTBU), No. 33 Fucheng Road, Haidian, Beijing, 100048, China.
J Sci Food Agric. 2017 Jan;97(2):434-443. doi: 10.1002/jsfa.7742. Epub 2016 May 11.
Ochratoxin A (OTA) poses a serious health hazard throughout the world and is often reported in food commodities. At present, biological approaches to detoxifying OTA are now widely considered to be the most promising methods.
In this study, a strain that was identified as Aspergillus niger was isolated, and it has a strong ability to detoxify OTA. The degradation product (compound 1) of OTA was treated with crude enzyme. The product has been isolated and identified as C H O Cl (ochratoxin α), which is a weak lipophilic molecule, in contrast to fat-soluble OTA. The cytotoxic response of compound 1 was revealed to be different to that of OTA. Compound 1 does not induce cellular oxidative damage in comparison with OTA, which may cause lipid peroxidation (MDA), reduce SOD activity and induce DNA damage.
This study indicates that A. niger has the ability to detoxify OTA. The OTA degradation product, ochratoxin α, does not exert cytotoxic effects on cell metabolism. A. niger has prospective uses for the OTA decontamination of food and agricultural fields. © 2016 Society of Chemical Industry.
赭曲霉毒素A（OTA）在全球范围内对健康构成严重危害，并且经常在食品中被检测到。目前，生物法去除OTA被广泛认为是最具前景的方法。
在本研究中，分离出了一株被鉴定为黑曲霉的菌株，它具有很强的去除OTA的能力。OTA的降解产物（化合物1）用粗酶处理。该产物已被分离并鉴定为C₁₇H₁₈ClNO₆（赭曲霉毒素α），与脂溶性的OTA相比，它是一种弱亲脂性分子。结果表明化合物1的细胞毒性反应与OTA不同。与可能导致脂质过氧化（MDA）、降低超氧化物歧化酶（SOD）活性并诱导DNA损伤的OTA相比，化合物1不会诱导细胞氧化损伤。
本研究表明黑曲霉具有去除OTA的能力。OTA降解产物赭曲霉毒素α对细胞代谢没有细胞毒性作用。黑曲霉在食品和农业领域OTA去污方面具有潜在用途。© 2016化学工业协会。
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