State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Toxins (Basel). 2022 Aug 20;14(8):569. doi: 10.3390/toxins14080569.
Ochratoxin A (OTA) is one of the most prevalent mycotoxins that threatens food and feed safety. Biodegradation of OTA has gained much attention. In this study, an strain named ANSA176, with a strong OTA-detoxifying ability, was isolated from donkey intestinal chyme and characterized. The strain ANSA176 could degrade 97.43% of 1 mg/mL OTA into OTα within 12 h, at 37 °C. The optimal levels for bacterial growth were 22-37 °C and pH 6.0-9.0. The effects of ANSA176 on laying hens with an OTA-contaminated diet were further investigated. A total of 36 laying hens were assigned to three dietary treatments: control group, OTA (250 µg/kg) group, and OTA + ANSA176 (6.2 × 10 CFU/kg diet) group. The results showed that OTA decreased the average daily feed intake (ADFI) and egg weight (EW); meanwhile, it increased serum alanine aminopeptidase (AAP), leucine aminopeptidase (LAP), β2-microglobulin (β2-MG), immunoglobulin G (IgG), tumor necrosis factor-α (TNF-α), and glutathione reductase (GR). However, the ANSA176 supplementation inhibited or attenuated the OTA-induced damages. Taken together, OTA-degrading strain ANSA176 was able to alleviate the immune injury and inflammation induced by OTA.
赭曲霉毒素 A（OTA）是一种最普遍的霉菌毒素，威胁着食物和饲料的安全。OTA 的生物降解引起了广泛关注。在本研究中，从驴肠食糜中分离出一株具有强 OTA 解毒能力的菌株 ANSA176，并对其进行了鉴定。该菌株 ANSA176 能在 37°C 下，12 小时内将 1mg/mL 的 OTA 降解为 97.43%的 OTα。细菌生长的最佳水平为 22-37°C 和 pH 值 6.0-9.0。进一步研究了 OTA 污染日粮对产蛋鸡的影响。将 36 只产蛋鸡分为三组：对照组、OTA（250μg/kg）组和 OTA+ANSA176（6.2×10 CFU/kg 日粮）组。结果表明，OTA 降低了平均日采食量（ADFI）和蛋重（EW）；同时，血清丙氨酸氨基肽酶（AAP）、亮氨酸氨基肽酶（LAP）、β2-微球蛋白（β2-MG）、免疫球蛋白 G（IgG）、肿瘤坏死因子-α（TNF-α）和谷胱甘肽还原酶（GR）升高。然而，ANSA176 补充剂抑制或减轻了 OTA 引起的损伤。综上所述，OTA 降解菌株 ANSA176 能够减轻 OTA 引起的免疫损伤和炎症。
