Bailleul C, Borrelly-Villereal M C, Chassaigne M, Ropars C
Centre Régional de Transfusion Sanguine, Centre National de la Recherche Scientifique-GS 84, Tours, France.
Biotechnol Appl Biochem. 1989 Feb;11(1):31-40. doi: 10.1111/j.1470-8744.1989.tb00049.x.
Internalization of inositol hexaphosphate (IHP) in mammalian red blood cells (RBC) produces a modification of the hemoglobin-oxygen affinity, leading to a rightward shift of the dissociation curve. The process of incorporation, based on an osmotic shock, has been tested on RBC of different species. Two dialysis protocols have been defined to transform RBC, the first one for small volumes in a cellulose bag and the other for larger volumes using a commercially available dialysis device. Different optimal conditions must be used for each species. Most of the cellular characteristics of the transformed RBC having encapsulated IHP are similar to those of native cells. For several species, such modified RBC could be reinfused and used for physiological studies.
哺乳动物红细胞（RBC）中肌醇六磷酸（IHP）的内化会导致血红蛋白与氧气亲和力的改变，从而使解离曲线向右移动。基于渗透休克的掺入过程已在不同物种的红细胞上进行了测试。已定义了两种透析方案来转化红细胞，第一种用于在纤维素袋中处理小体积样本，另一种用于使用市售透析设备处理大体积样本。每个物种必须使用不同的最佳条件。封装了IHP的转化红细胞的大多数细胞特征与天然细胞相似。对于几种物种，这种经过修饰的红细胞可以重新注入并用于生理学研究。
