Walker Heather J, Steels Chloe, Bendell Lilias, Clench Malcolm R, Read David J, Cameron Duncan D, Burrell Michael M
Dept. Animal and Plant Sciences, Sheffield University, Western Bank, Sheffield, UK.
Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield, UK.
Proteomics. 2016 Jun;16(11-12):1690-4. doi: 10.1002/pmic.201500493. Epub 2016 May 27.
Arginine is an important amino acid but has been barely studied in plants. The little research that has been done indicates that the pathways of synthesis are similar to those found in animals and procaryotes. However little is known about the cellular and tissue localization of the amino acid in plants. The research reported in this paper was designed to examine whether MALDI-MSI was sufficiently sensitive to examine the distribution of this amino acid in plant material, and whether the synthetic pathways were co-located. In wheat and orchid roots, the amount of arginine in tissues varies greatly and the pathways for its synthesis were not always detected with the amino acid.
精氨酸是一种重要的氨基酸，但在植物中却鲜有研究。已开展的少量研究表明，其合成途径与在动物和原核生物中发现的途径相似。然而，关于这种氨基酸在植物中的细胞和组织定位却知之甚少。本文所报道的研究旨在检验基质辅助激光解吸/电离质谱成像（MALDI-MSI）是否足够灵敏，以检测这种氨基酸在植物材料中的分布，以及其合成途径是否位于同一位置。在小麦和兰花的根中，组织中的精氨酸含量差异很大，而且其合成途径并非总能通过该氨基酸检测到。
