Anderson David M G, Carolan Vikki A, Crosland Susan, Sharples Kate R, Clench Malcolm R
Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, UK.
Rapid Commun Mass Spectrom. 2009 May;23(9):1321-7. doi: 10.1002/rcm.3973.
Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) has been used to image the distribution of the pesticide nicosulfuron (2-[[(4,6-dimethoxypyrimidin-2-yl)aminocarbonyl]aminosulfonyl]-N,N-dimethyl-3-pyridinecarboxamide) in plant tissue using direct tissue imaging following root and foliar uptake. Sunflower plants inoculated with nicosulfuron were horizontally sectioned at varying distances along the stem in order to asses the extent of translocation; uptake via the leaves following foliar application to the leaves and uptake via the roots from a hydroponics system were compared. An improved sample preparation methodology, encasing samples in ice, allowed sections from along the whole of the plant stem from the root bundle to the growing tip to be taken. Images of fragment ions and alkali metal adducts have been generated that show the distribution of the parent compound and a phase 1 metabolite in the plant. Positive and negative controls have been included in the images to confirm ion origin and prevent false-positive results which could originate from endogenous compounds present within the plant tissue.
基质辅助激光解吸/电离质谱成像（MALDI-MSI）已被用于通过根和叶吸收后的直接组织成像来观察农药烟嘧磺隆（2-[[(4,6-二甲氧基嘧啶-2-基)氨基羰基]氨基磺酰基]-N,N-二甲基-3-吡啶甲酰胺）在植物组织中的分布。对接种了烟嘧磺隆的向日葵植株沿茎在不同距离处进行水平切片，以评估转运程度；比较了叶面喷施后通过叶片的吸收以及从水培系统通过根部的吸收。一种改进的样品制备方法，即将样品包裹在冰中，使得能够从根部束到生长顶端沿着整个植物茎部采集切片。已生成碎片离子和碱金属加合物的图像，这些图像显示了母体化合物和一种一期代谢物在植物中的分布。图像中包含了阳性和阴性对照，以确认离子来源并防止可能源于植物组织中内源性化合物的假阳性结果。
