Silva-Junior José Audenor, de Souza França Daniele, Moraes Ramiris César Souza, Gaiotto Fernanda Amato
Pós-graduação em Genética e Biologia Molecular, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil.
Pós-graduação em Ecologia e Conservação da Biodiversidade, Universidade Estadual de Santa Cruz (UESC), Rodovia Ilhéus-Itabuna km 16 s/n, Salobrinho, Ilhéus, Bahia, 45662-900, Brazil.
Mol Biol Rep. 2016 Jun;43(6):451-5. doi: 10.1007/s11033-016-3981-3. Epub 2016 Apr 9.
Manilkara maxima is an endemic tree species of the Atlantic Forest in southern Bahia, Brazil. It is considered important for forest conservation due to its mutualistic interactions with endemic and endangered animals. Our aim was to develop microsatellite markers to estimate genetic diversity in order to provide information for effectiveness of future conservation programs. We used next generation sequencing technology to develop the first specific microsatellite markers for M. maxima. Seventeen new microsatellite loci were applied in 72 individuals sampled in three natural populations. On average, the number of alleles per loci was 8.8. The expected heterozygosity varied between 0.72 and 0.77, indicating that the developed set of molecular markers is useful for genetic diversity studies. Additionally, the estimated value for the combined probability of exclusion (Q) was greater than 0.999, which indicates the powerful of these molecular tools for paternity and kinship analysis. Our results demonstrate that the set of microsatellites developed in this work is a powerful tool for population genetics, molecular ecology and conservation biology purposes.
大果铁线子是巴西巴伊亚州南部大西洋森林的一种特有树种。由于它与当地特有和濒危动物存在互利共生关系，因而被认为对森林保护具有重要意义。我们的目标是开发微卫星标记以估计遗传多样性，从而为未来保护计划的有效性提供信息。我们利用下一代测序技术开发了首个针对大果铁线子的特异性微卫星标记。17个新的微卫星位点应用于从三个自然种群中采集的72个个体。每个位点的等位基因平均数为8.8。预期杂合度在0.72至0.77之间，这表明所开发的这套分子标记对于遗传多样性研究是有用的。此外，估计的累积排除概率（Q）值大于0.999，这表明这些分子工具在亲子鉴定和亲属关系分析方面的强大作用。我们的结果表明，本研究中开发的这套微卫星标记是用于种群遗传学、分子生态学和保护生物学目的的有力工具。
