Centro de Biotecnologia e Genética , Universidade Estadual de Santa Cruz (UESC) , Rodovia Ilhéus-Itabuna km 16s/n, Salobrinho, 45662-900 Ilhéus, Bahia , Brazil.
AoB Plants. 2013;5:plt006. doi: 10.1093/aobpla/plt006. Epub 2013 Mar 13.
Manilkara multifida is a tropical tree that is endemic to the Atlantic forests of southern Bahia, Brazil. Currently, populations of this species are restricted to fragmented landscapes that are susceptible to anthropogenic disturbances. Considering this issue, and that there is no genetic information available for this endangered species, we developed microsatellite markers for M. multifida to provide resources for future conservation genetics studies. Using an enriched genomic library, we isolated eight polymorphic microsatellite loci and optimized the amplification conditions for M. multifida. For each locus, we estimated the number of alleles, H E and H O, paternity exclusion Q, individual identity I and fixation index F, and examined the presence of null alleles. The mean number of alleles was 11.9, and the heterozygosity was high at all loci (average H E = 0.809 and H O = 0.777). The combined values for both paternity exclusion and individual identity were Q = 0.9959 and I = 5.45 × 10(-11), respectively. No evidence of null alleles was detected. The results of our analysis indicated that all eight microsatellites are promising for assessing questions involving inbreeding, gene flow, co-ancestry and mating patterns in M. multifida.
聚果榕是一种生长于巴西巴伊亚州南部大西洋森林的热带树种。目前，该物种的种群局限于易受人为干扰的破碎景观中。考虑到这一问题，并且该濒危物种尚无遗传信息，我们开发了聚果榕的微卫星标记，为未来的保护遗传学研究提供资源。使用富集基因组文库，我们分离出了 8 个多态性微卫星位点，并优化了聚果榕的扩增条件。对于每个位点，我们估计了等位基因数、H E 和 H O、父系排除 Q、个体身份 I 和固定指数 F，并检查了是否存在无效等位基因。等位基因数的平均值为 11.9，所有位点的杂合度都很高（平均 H E = 0.809，H O = 0.777）。父系排除和个体身份的综合值分别为 Q = 0.9959 和 I = 5.45 × 10(-11)。未检测到无效等位基因的证据。我们的分析结果表明，这 8 个微卫星标记均适合评估聚果榕的近交、基因流、共同血统和交配模式等问题。
