Geeves Michael A
School of Biosciences, University of Kent, Canterbury, UK.
Biopolymers. 2016 Aug;105(8):483-91. doi: 10.1002/bip.22853.
Myosins are a large family of molecular motors that use the common P-loop, Switch 1 and Switch 2 nucleotide binding motifs to recognize ATP, to create a catalytic site than can efficiently hydrolyze ATP and to communicate the state of the nucleotide pocket to other allosteric binding sites on myosin. The energy of ATP hydrolysis is used to do work against an external load. In this short review I will outline current thinking on the mechanism of ATP hydrolysis and how the energy of ATP hydrolysis is coupled to a series of protein conformational changes that allow a myosin, with the cytoskeleton track actin, to operate as a molecular motor of distinct types; fast movers, processive motors or strain sensors. © 2016 Wiley Periodicals, Inc. Biopolymers 105: 483-491, 2016.
肌球蛋白是一个庞大的分子马达家族，它们利用常见的P环、开关1和开关2核苷酸结合基序来识别ATP，形成一个能够高效水解ATP的催化位点，并将核苷酸口袋的状态传递给肌球蛋白上的其他变构结合位点。ATP水解产生的能量用于克服外部负载做功。在这篇简短的综述中，我将概述目前关于ATP水解机制的观点，以及ATP水解的能量如何与一系列蛋白质构象变化相偶联，这些变化使得肌球蛋白能够与细胞骨架肌动蛋白一起，作为不同类型的分子马达发挥作用，如快速移动者、持续运动马达或应变传感器。© 2016威利期刊公司。生物聚合物105: 483 - 491, 2016年。
