Zheng Hua, Qin Xia, Song Hui, Tang Chao-lin, Ruan Jun-xiang, Zhang Hong-ye, Lu Shi-yin, Liang Yong-hong, Su Zhi-heng
Zhongguo Zhong Yao Za Zhi. 2015 Oct;40(20):4088-93.
To study the anti-coagulant effect and influence of danggui Sini decoction (DSD) on rat's plasma endogenous metabolites by animal experiment and ¹H-NMR based metabolomics method. After intragastric administration of Danggui Sini Decoction for 7 days, Plasma thrombin time (TT) was measured. Rat plasma metabolic fingerprint in two groups was analyzed using ¹H-NMR, based on which the principal component analysis( PCA) and orthogonal partial least-squares discriminant analysis(OPLS-DA) models for metabonomic analysis. Potential biomarkers were screened by using variable importance in the projection (VIP) and T test. DSD could prolong TT of the rat significantly (P < 0.05). Compared with control group, six kinds of endogenous metabolites in DSD group change significantly (P < 0.05), among which isobutyrate, carnitine and phenylalanine content had an upward trend (P < 0.01) and lysine, Histidine and cholesterol content had a downward trend (P < 0.05). It is likely that carnitine, phenylalanine, Histidine and cholesterol are the potential metabolic markers in the anti-coagulant process and DSD affects the platelet aggregation and the expression of tissue factor and fiber protease by regulating the energy, amino acid and lipid metabolism.
通过动物实验和基于¹H-NMR的代谢组学方法，研究当归四逆汤（DSD）对大鼠血浆内源性代谢物的抗凝作用及影响。给予当归四逆汤灌胃7天后，测定血浆凝血酶时间（TT）。采用¹H-NMR分析两组大鼠血浆代谢指纹图谱，在此基础上建立代谢组学分析的主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）模型。利用投影变量重要性（VIP）和T检验筛选潜在生物标志物。DSD可显著延长大鼠TT（P<0.05）。与对照组相比，DSD组6种内源性代谢物有显著变化（P<0.05），其中异丁酸、肉碱和苯丙氨酸含量呈上升趋势（P<0.01），赖氨酸、组氨酸和胆固醇含量呈下降趋势（P<0.05）。肉碱、苯丙氨酸、组氨酸和胆固醇可能是抗凝过程中的潜在代谢标志物，DSD可能通过调节能量、氨基酸和脂质代谢影响血小板聚集及组织因子和纤维蛋白酶的表达。
