Kitano H, Yan C H, Maeda Y, Ise N
Biopolymers. 1989 Mar;28(3):693-702. doi: 10.1002/bip.360280302.
The binary association process was further examined by direct visual observation using a fluorescence microscope connected to an image-processing system and by a spectrophotometric method. In the present paper, we used human serum albumin (HSA)- and fragmented anti-HSA antibody-carrying fluorescent latex particles in order to attain higher sensitivities and exclude complexities that might be caused by dissociation of the association product. The rate constant of the binary association process was estimated to be 6.0 x 10(8) M-1 s-1 at 30 degrees C and pH 7.6. The activation parameters of the association process were evaluated. The effects of pH and viscosity of the suspension on the association process were also examined.
通过使用连接到图像处理系统的荧光显微镜进行直接视觉观察以及分光光度法，对二元缔合过程进行了进一步研究。在本文中，我们使用了携带人血清白蛋白（HSA）和抗HSA抗体片段的荧光乳胶颗粒，以便获得更高的灵敏度并排除缔合产物解离可能引起的复杂性。在30℃和pH 7.6条件下，二元缔合过程的速率常数估计为6.0×10⁸ M⁻¹ s⁻¹。评估了缔合过程的活化参数。还研究了悬浮液的pH和粘度对缔合过程的影响。
