Hao Li-Jing, Zheng Wen-Jiao, Wang Shu-Fen, Zheng Ying, He Shao-Heng, Zhang Bin
Department of Dentistry, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2016 Mar;36(3):297-302.
To investigate the expression of LRG-1 in clinical specimens and Tca8113 cell line of tongue carcinoma and analyze the relationship between LRG-1 expression and the clinicopathological parameters.
LRG-1 expression was detected in 40 tongue squamous cell carcinoma (TSCC) tissues and paired normal adjacent tissues, 20 atypical hyperplasia tissues of the tongue, and 20 tissues of tongue cancer in situ using immunohistochemical method. The expression of LRG-1 in Tca8113 cell line was detected using flow cytometry. The expression of LRG-1 was also detected in human TSCC tissues and Tca8113 cells with Western blotting. The effect of LRG-1 on the proliferation of HUVECs was determined using MTT assay, and its effect on angiogenesis was evaluated with Matrigel tube formation assays.
Human TSCC tissues had a significantly higher rate of positive expression for LRG-1 (85%, 34/40) than the adjacent tissues (10%, 4/40), invasive tongue cancer (30%, 6/20), and tongue cancer in situ (50%, 10/20) (P<0.05). LRG-1 expression was correlated with the degree of tumor differentiation, clinical stage and lymph node metastasis of the tumor (P<0.05) but not with the patients' age or gender. In the in vitro experiment, LRG-1 promoted HUVEC proliferation and angiogenesis.
Abnormal LRG-1 expression is present in the human TSCC tissue and Tca8113 cells. LRG-1 can promote HUVEC proliferation and angiogenesis in vitro, suggesting its possible role in promoting tumor angiogenesis.
研究富含亮氨酸α2糖蛋白1（LRG-1）在舌癌临床标本及Tca8113细胞系中的表达情况，并分析LRG-1表达与临床病理参数之间的关系。
采用免疫组织化学方法检测40例舌鳞状细胞癌（TSCC）组织及其配对的癌旁正常组织、20例舌非典型增生组织和20例舌原位癌组织中LRG-1的表达。采用流式细胞术检测Tca8113细胞系中LRG-1的表达。同时采用蛋白质免疫印迹法检测人TSCC组织及Tca8113细胞中LRG-1的表达。采用MTT法检测LRG-1对人脐静脉内皮细胞（HUVECs）增殖的影响，并用基质胶管形成实验评估其对血管生成的作用。
人TSCC组织中LRG-1阳性表达率（85%，34/40）显著高于癌旁组织（10%，4/40）、浸润性舌癌（30%，6/20）和舌原位癌（50%，10/20）（P<0.05）。LRG-1表达与肿瘤分化程度、临床分期及肿瘤淋巴结转移相关（P<0.05），但与患者年龄和性别无关。体外实验中，LRG-1促进HUVECs增殖和血管生成。
人TSCC组织及Tca8113细胞中存在LRG-1异常表达。LRG-1在体外可促进HUVECs增殖和血管生成，提示其可能在促进肿瘤血管生成中发挥作用。
