通过纳米级铋减少口腔生物膜形成细菌的新策略。
New Strategy of Reducing Biofilm Forming Bacteria in Oral Cavity by Bismuth Nanoparticles.
机构信息
Student Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Oral and Dental Disease Research Center, Department of Oral & Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Biomed Res Int. 2021 Feb 2;2021:6695692. doi: 10.1155/2021/6695692. eCollection 2021.
OBJECTIVE
and are the most important species in dental decay and producing biofilm. Treatment with chlorhexidine 2% mouthwash for 7 days is the best way to eliminate these bacteria. However, due to the ability of these bacteria to survive in harsh environments, increasing emergence of bacterial resistance against available antibiotics, and favorable properties of nanoparticles including broad spectrum antimicrobial activity and lower toxicity, we decided to evaluate reducing biofilm forming bacteria in oral cavity by bismuth nanoparticles.
MATERIALS AND METHODS
This was a cross-sectional study of 40 samples isolated from the patients visiting dental clinics in Shiraz in 2019. Samples, which showed growth, were cultured on blood agar plates and incubated for the PCR procedure. Nanoparticle powder was dissolved in high-purity water, and the final concentration of bismuth nanoparticles (BiNPs) was measured with a spectrophotometer. Minimum inhibitory concentration (MIC) of BiNPs against and was determined by the microbroth dilution method according to methods for antimicrobial susceptibility tests. Also, bactericidal assays were conducted in a Mueller-Hinton broth medium and reported as the concentration of BiNPs that reduced the viable bacterial count by 99.9%. Statistical analysis was carried out using SPSS 21 and one-way analysis of variance, and values less than 0.05 were considered significant.
RESULTS
MICs of BiNP suspension against and were 2.5 and 5 g/ml, respectively. Minimum bactericidal concentrations (MBC) of BiNP suspension against and were 5 and 10 g/ml, respectively. Antibacterial activity of BiNPs was compared with chlorhexidine 2%. MICs of BiNPs against and were one-twentieth less than those of chlorhexidine. MBC of BiNPs against both pathogens was one-tenth less than those of chlorhexidine.
CONCLUSION
BiNPs were more effective than chlorhexidine, and MIC and MBC of bismuth nanoparticles are lower than those of chlorhexidine.
目的
和 是导致龋齿和生物膜形成的最重要的物种。用 2%洗必泰漱口水治疗 7 天是消除这些细菌的最佳方法。然而，由于这些细菌能够在恶劣环境中生存，对现有抗生素的细菌耐药性不断增加，以及纳米粒子具有广谱抗菌活性和较低毒性等优良特性，我们决定评估纳米级的铋粒子对口腔生物膜形成细菌的减少作用。
材料和方法
这是 2019 年在设拉子牙科诊所就诊的 40 例患者分离样本的横断面研究。在血琼脂平板上培养显示生长的样本，并进行 PCR 程序。纳米粒子粉末溶解在高纯水中，并用分光光度计测量纳米级铋粒子（BiNPs）的最终浓度。根据抗菌药物敏感性试验方法，采用微量肉汤稀释法测定 BiNPs 对 和 的最小抑菌浓度（MIC）。同时，在 Mueller-Hinton 肉汤培养基中进行杀菌试验，并报告减少活菌计数 99.9%的 BiNPs 浓度。采用 SPSS 21 进行统计学分析，采用单因素方差分析， 0.05。
结果
BiNP 混悬液对 和 的 MIC 分别为 2.5 和 5g/ml。BiNP 混悬液对 和 的最低杀菌浓度（MBC）分别为 5 和 10g/ml。BiNPs 的抗菌活性与洗必泰 2%相比。BiNPs 对 和 的 MIC 比洗必泰低二十分之一。BiNPs 对两种病原体的 MBC 比洗必泰低十分之一。
结论
BiNPs 比洗必泰更有效，BiNPs 的 MIC 和 MBC 均低于洗必泰。
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