Jensen Henriette Stokbro, Sekar Raju, Shepherd Will J, Osborn Andrew M, Tait Simon, Biggs Catherine A
Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK.
Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, China.
Microbiologyopen. 2016 Aug;5(4):616-25. doi: 10.1002/mbo3.356. Epub 2016 Apr 10.
This study describes the temporal and spatial variability of bacterial communities within a combined sewer system in England. Sampling was conducted over 9 months in a sewer system with intensive monitoring of hydraulic conditions. The bacterial communities were characterized by 16S rRNA gene-targeted terminal restriction fragment length polymorphism analysis. These data were related to the hydraulic data as well as the sample type, location, and time. Temporal and spatial variation was observed between and within wastewater communities and biofilm communities. The bacterial communities in biofilm were distinctly different from the communities in wastewater and exhibited greater spatial variation, while the wastewater communities exhibited variability between different months of sampling. This study highlights the variation of bacterial communities between biofilm and wastewater, and has shown both spatial and temporal variations in bacterial communities in combined sewers. The temporal variation is of interest for in-sewer processes, for example, sewer odor generation, as field measurements for these processes are often carried out over short durations and may therefore not capture the influence of this temporal variation of the bacterial communities.
本研究描述了英格兰某合流制排水系统中细菌群落的时空变异性。在一个对水力条件进行密集监测的排水系统中进行了为期9个月的采样。通过靶向16S rRNA基因的末端限制性片段长度多态性分析对细菌群落进行表征。这些数据与水力数据以及样品类型、位置和时间相关。在废水群落和生物膜群落之间以及内部均观察到了时空变化。生物膜中的细菌群落与废水中的群落明显不同，且表现出更大的空间变异性，而废水群落在不同采样月份之间表现出变异性。本研究突出了生物膜和废水之间细菌群落的差异，并显示了合流制下水道中细菌群落的时空变化。这种时间变化对于下水道内的过程很重要，例如下水道气味的产生，因为这些过程的现场测量通常在短时间内进行，因此可能无法捕捉到细菌群落这种时间变化的影响。
