Zhang S M, Li J X, Wei Y M, Yang Z M
Department of Biochemistry, Second Military Medical College, Shanghai, People's Republic of China.
Biomed Chromatogr. 1989 Jan;3(1):10-3. doi: 10.1002/bmc.1130030104.
A simplified method for the preparation of wheat germ agglutinin(WGA)-Sepharose 4B by coupling highly purified WGA, prepared by improved affinity chromatography, with BrCN activated Sepharose 4B in a solution of high carbonate buffer is described. The amount of WGA linked to Sepharose 4B was 82.40% (3.07 mg WGA per ml Sepharose 4B). MN blood group antigens of human erythrocyte membranes purified with WGA-Sepharose 4B affinity chromatography showed a single band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE). The yield of the antigens from 400 mL fresh blood was 32-40 mg. The WGA-Sepharose 4B column could be used several times without loss of activity.
本文描述了一种制备麦胚凝集素（WGA）-琼脂糖4B的简化方法，该方法是在高碳酸盐缓冲溶液中，将通过改进的亲和色谱法制备的高纯度WGA与溴化氰活化的琼脂糖4B偶联。与琼脂糖4B结合的WGA量为82.40%（每毫升琼脂糖4B含3.07毫克WGA）。用WGA-琼脂糖4B亲和色谱法纯化的人红细胞膜MN血型抗原在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳（SDS/PAGE）上显示出一条单一的条带。从400毫升新鲜血液中获得的抗原产量为32 - 40毫克。WGA-琼脂糖4B柱可重复使用多次而不失活。
