麦胚凝集素（WGA）是一种糖基化蛋白吗？

Is wheat germ agglutinin (WGA) a glycosylated protein?

作者信息

Kisiel D G, Gindzieński A, Zwierz K

机构信息

Department of General and Organic Chemistry, Medical Academy of Białystok.

出版信息

Rocz Akad Med Bialymst. 1996;41(2):284-92.

PMID:9020540

Abstract

Lectin isolated from wheat germ was purified by affinity chromatography on ovogel column, recrystallization and gel filtration on Ultrogel AcA 44 column, eluted with borate buffer to eliminate accidental lectin-ligand interaction products. In 87-times purified WGA preparation fucose, xylose, mannose, galactose and glucose were found, amounting 1.5% of lectin mass.

摘要

从小麦胚中分离得到的凝集素通过在卵黄球蛋白柱上进行亲和层析、重结晶以及在Ultrogel AcA 44柱上进行凝胶过滤来纯化，用硼酸盐缓冲液洗脱以消除偶然产生的凝集素-配体相互作用产物。在经过87次纯化的麦胚凝集素制剂中发现了岩藻糖、木糖、甘露糖、半乳糖和葡萄糖，其含量占凝集素质量的1.5%。