Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, China.
Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China.
Analyst. 2016 May 10;141(10):2861-4. doi: 10.1039/c6an00160b.
Orderly nucleic acid aggregates (ONAAs) self-assembled on mesoporous silica nanoparticles (MSNs) with positively charged aminopropyl groups (PC) were firstly developed. Interestingly, a novel electrostatic DNA self-assembly could realize hybridization chain reaction (HCR) on the surface of PCMSNs in single cells. Significantly, a non-destructive amplification strategy based on ONAAs-PCMSNs was successfully developed for miRNA detection and in situ imaging by the prominent and agminated fluorescence-bright spots in living cells.
有序核酸聚集体（ONAAs）首次在带有正电荷的氨丙基基团（PC）的介孔硅纳米粒子（MSNs）上自组装。有趣的是，一种新型静电 DNA 自组装可以在单个细胞的 PCMSNs 表面上实现杂交链式反应（HCR）。重要的是，通过在活细胞中明显且聚集的荧光亮点，成功开发了基于 ONAAs-PCMSNs 的非破坏性扩增策略，用于 miRNA 检测和原位成像。
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