Zhang Zhen, Wang Yuanyuan, Zhang Ningbo, Zhang Shusheng
Shandong Province Key Laboratory of Detection Technology for Tumor Makers , College of Chemistry and Chemical Engineering , Linyi University , Linyi 276000 , P. R. China . Email:
Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong , Shandong Normal University , Jinan 250014 , P. R. China.
Chem Sci. 2016 Jul 1;7(7):4184-4189. doi: 10.1039/c6sc00694a. Epub 2016 Mar 7.
We herein report a novel finding that nucleic acid molecular aggregates (NAMAs) self-assembled on graphene oxide nanoplates (GONPs) as a result of DNA rolling circle amplification (RCA) and a functionalized triple-helix probe (THP) in single cells. The functionalized THP containing the aptamer region for target recognition and the trigger DNA region for RCA was firstly used to activate RCA for miRNA imaging in single cells. Interestingly, NAMAs with the fluorescent labels were hybridized by both the RCA products and FAM-DNA, and could partly self-assemble on GONPs; meanwhile, NAMAs could extend from the GONPs, which led to the quenched fluorescence being renewed. Significantly, the NAMAs were successfully applied for low-abundance miRNA detection and imaging in single cells. The self-assembled NAMAs could generate prominent and agminated fluorescence-bright spots in single cancer cells, which will effectively drive cell imaging into a new era.
我们在此报告一项新发现，即由于DNA滚环扩增（RCA）和单细胞中的功能化三链体探针（THP），核酸分子聚集体（NAMAs）在氧化石墨烯纳米片（GONPs）上自组装。含有用于靶标识别的适体区域和用于RCA的触发DNA区域的功能化THP首先用于激活RCA以进行单细胞中的miRNA成像。有趣的是，带有荧光标记的NAMAs与RCA产物和FAM-DNA都杂交，并能部分自组装在GONPs上；同时，NAMAs可以从GONPs延伸，这导致淬灭的荧光得以恢复。值得注意的是，NAMAs成功应用于单细胞中低丰度miRNA的检测和成像。自组装的NAMAs可以在单个癌细胞中产生突出且聚集的荧光亮点，这将有效地推动细胞成像进入一个新时代。
