Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, PR China.
ACS Appl Mater Interfaces. 2012 Dec;4(12):6450-3. doi: 10.1021/am302268t. Epub 2012 Dec 4.
A new enzyme-free signal amplification-based assay for microRNA (miRNA) detection is developed by using hybridization chain reaction (HCR) coupled with a graphene oxide (GO) surface-anchored fluorescence signal readout pathway. MiRNAs can efficiently initiate HCR between two species of fluorescent hairpin probes. After HCR, both of the excess hairpin probes and the HCR products will be anchored on the GO surface. The fluorescence of the hairpin probes can be completely quenched by GO, whereas the HCR products maintain strong fluorescence. Therefore, integrating HCR strategy for signal amplification with selective fluorescence quenching effects of GO provides a versatile miRNA assay.
一种新的基于无酶信号放大的微 RNA（miRNA）检测方法是通过杂交链式反应（HCR）与氧化石墨烯（GO）表面锚定的荧光信号读出途径相结合而开发的。miRNA 可以有效地在两种荧光发夹探针之间引发 HCR。HCR 后，多余的发夹探针和 HCR 产物都将被锚定在 GO 表面上。GO 可以完全猝灭发夹探针的荧光，而 HCR 产物则保持强荧光。因此，将 HCR 策略用于信号放大与 GO 的选择性荧光猝灭效应相结合，为 miRNA 检测提供了一种通用的方法。
